Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/26865

TítuloPotential of marine sponge collagen coatings for skin regeneration strategies
Autor(es)Silva, Joana M.
Silva, Tiago H.
Prata, Margarida Barros
Cerqueira, M. T.
Pirraco, Rogério
Giovine, M.
Marques, A. P.
Reis, R. L.
Palavras-chaveCoatings
Cytotoxicity
Marine collagen
Skin regeneration
DataOut-2013
EditoraWiley-Blackwell
CitaçãoMoreira-Silva J., Silva T. H., Prata M. B., Cerqueira M. T., Pirraco R. P., Giovine M., Marques A. P., Reis R. L. Potential of marine sponge collagen coatings for skin regeneration strategies, Journal of Tissue Engineering and Regenerative Medicine, Vol. 7, Issue s1, pp. 33, doi:doi:10.1002/term.1822, 2013
Resumo(s)Collagen is the most abundant protein in mammals and has found a wide range of applications in human health-related fields, namely cosmetics, pharmaceutical, dental, skin regeneration, ophthalmology, cardiac surgery and orthopaedics. Bovine and porcine bones and skins are the main industrial sources of collagen. However, due to religious constrains on the use of porcine products and to the risk of bovine spongiform encephalopathy (BSE) and other diseases posing to humans when infected bovine derived products are used, other sources of collagen are being pursued. Marine raw-materials have arisen on the last years and, in particular, marine sponges can be an important source of collagen. In this work, collagen was extracted from the marine sponge Chondrosia reniformis using different extraction methods: (i) 0.5 M acetic acid with 10% pepsin; (ii) 50 mM Tris-HCl with 1M NaCl ; and (iii) 100 mM Tris-HCl, 10 mM EDTA, 8 M urea and 100 mM 2-mercaptoethanol. Cytotoxicity of the extracted collagen was assessed with L929 cell culture onto collagen coatings (5.5µg/ml), with evaluation of metabolic activity (MTS assay) and cell proliferation (DNA quantification). Coatings of collagen extracted from C. reniformis are not cytotoxic and promoted proliferation. Moreover, C. reniformis collagen has been characterized and identified as mainly of type IV, thus promising application in epidermal regeneration strategies. In particular, its role on the selection of the rapid proliferating keratinocytes is addressed
TipoconferenceAbstract
DescriçãoPublicado em "Journal of Tissue Engineering and Regenerative Medicine, vol. 7, supp. 1 (2013)
URIhttp://hdl.handle.net/1822/26865
Versão da editorahttp://onlinelibrary.wiley.com/doi/10.1002/term.2013.7.issue-s1/issuetoc
Arbitragem científicayes
AcessorestrictedAccess
Aparece nas coleções:3B’s - Resumos em livros de atas de conferências - indexados no ISI Web of Science

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