Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/26707
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Correia, Tiago R. | - |
dc.contributor.author | Antunes, Bernardo P. | - |
dc.contributor.author | Castilho, Pedro H. | - |
dc.contributor.author | Nunes, José C. | - |
dc.contributor.author | Amorim, M. T. Pessoa de | - |
dc.contributor.author | Escobar, I. C. | - |
dc.contributor.author | Correia, I. J. | - |
dc.contributor.author | Morão, A. | - |
dc.date.accessioned | 2013-12-05T11:30:10Z | - |
dc.date.available | 2013-12-05T11:30:10Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 1383-5866 | por |
dc.identifier.uri | https://hdl.handle.net/1822/26707 | - |
dc.description.abstract | The demanding ever-increasing quantities of highly purified biomolecules by bio-industries, has triggered the development of new, more efficient, purification techniques. The application of membrane-based technologies has become very attractive in this field, for their high throughput capability, simplicity of operation and scale-up. Herein we report the production of a bi-layer membrane by electrospinning (ES), in which a support of poly ε-caprolactone nanofibers was coated with a polyethylene oxide/sodium alginate layer, and subsequently cross-linked with calcium chloride. The membranes were characterized by SEM, ATR-FTIR, contact angle measurements, and were applied in the recovery process of a plasmid. The results show that membranes retained the suspended solids while allowing the permeation of plasmid DNA, with high recovery yields and improved RNA retention. Moreover, they also showed a very low fouling tendency. To the best of our knowledge it is the first time that ES membranes are applied in this type of bioprocess. | por |
dc.description.sponsorship | This work was supported by the Portuguese Foundation for Science and Technology (FCT), (PTDC/EME-TME/103375/2008 and PTDC/EBB-BIO/114320/2009). To Ricardo Fradique for helping in the production of the graphical abstract. | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.rights | openAccess | por |
dc.subject | Microfiltration | por |
dc.subject | Electrospinning | por |
dc.subject | Bi-layer membrane | por |
dc.subject | Lysate | por |
dc.subject | Plasmid DNA | por |
dc.subject | DNA | por |
dc.subject | Plasmid | por |
dc.title | A bi-layer electrospun nanofiltration membrane for plasmid DNA recovery from fermentation broths | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://dx.doi.org/10.1016/j.seppur.2013.03.049 | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 20 | por |
oaire.citationEndPage | 25 | por |
oaire.citationTitle | Separation and Purification Technology | por |
oaire.citationVolume | 112 | por |
dc.identifier.doi | 10.1016/j.seppur.2013.03.049 | - |
dc.subject.wos | Science & Technology | por |
sdum.journal | Separation and Purification Technology | por |
Aparece nas coleções: | DET/2C2T - Artigos em revistas internacionais com arbitragem científica |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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1-s2.0-S1383586613001962-main.pdf | 1,61 MB | Adobe PDF | Ver/Abrir |