Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/2616

TitleEvolution of ochratoxin A content from must to wine in Port wine microvinification
Author(s)Ratola, N.
Abade, E.
Simões, T.
Venâncio, Armando
Alves, Arminda
KeywordsOchratoxin A
Microvinification
Must
Port Wine
HPLC
Issue date2005
PublisherSpringer Verlag
JournalAnalytical and bioanalytical chemistry
Citation"Analytical and bioanalytical chemistry". ISSN 1618-2642. 382:2 (2005) 405–411.
Abstract(s)To study the evolution of ochratoxin A (OTA) content from must to wine during the making of Port Wine, grapes from the five most common varieties of Port Wine were harvested and combined in equal percentages in order to perform microvinifications. Three sets of assays were studied: a blank (A), where the most common Port Wine-making process was used; in the second (B), a solution of OTA was added to the initial must; in the third (C), the grapes were aspersed with an inoculating solution of OTA-producing fungi. Samples were collected, in duplicate, on four different occasions throughout the process. The influence of the addition of SO_2 to the must was also assessed in each set. The quantification of OTA was based on the standard reference method for wines (European Standard prEN 14133), which includes clean-up via immunoaffinity columns and HPLC with fluorescence detection. The limits of detection were 0.076 μg/l for wine and 0.114 μg/l for must. The method was validated by assessing the precision, accuracy and by obtaining an estimate of the global uncertainty. Overall, the levels of OTA observed during the vinifications dropped by up to 92%, and no grapes used in this work were contaminated naturally.
TypeArticle
URIhttp://hdl.handle.net/1822/2616
DOI10.1007/s00216-005-3176-6
ISSN1618-2642
1618-2650
Publisher versionhttps://link.springer.com/article/10.1007%2Fs00216-005-3176-6
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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