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https://hdl.handle.net/1822/26104
Título: | Hydroxylation of polypropylene using the monooxygenase mutant 139-3 from Bacillus megaterium BM3 |
Autor(es): | Hasmann, Andrea Eberl, Anita Schneider, Konstantin Schoening, K.-U. Kaufmann, Franz Glieder, Anton Kovac, Janez Paulo, Artur Cavaco Wehrschuetz-Sigl, Eva Guebitz, G. M. |
Palavras-chave: | Oxidoreductase Polypropylene Polymer Surface modification |
Data: | 2012 |
Editora: | Informa Healthcare |
Revista: | Biocatalysis and Biotransformation |
Resumo(s): | Enzymatic hydroxylation of polypropylene (PP) was investigated in order to increase hydrophilicity. A mutant (139-3) of the P450monooxygenase from Bacillus megaterium expressed in E. coli DH5α was purified using anion exchange chromatography. Hydroxylation of PP fabrics led to a dramatic increase of hydrophilicity as indicated by a water drop dissipation time of below 1 s compared to the hydrophobic reference material. Likewise, a 4.9 cm increase of rising height was measured which remained consistent after 144 h of storage. Similarly, enzymatic hydroxylation of PP films lead to a decrease of the WCA from 104.6° to 77.3° with no major change after exposure to air for 6 days. Using X-ray photoelectron spectroscopy, an increase in normalized atomic concentrations of oxygen from 1.40 to 4.98% for the CO-inhibited and enzyme treated sample, respectively, was measured confirming enzymatic hydroxylation. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/26104 |
DOI: | 10.3109/10242422.2012.645367 |
ISSN: | 1024-2422 1029-2446 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | DET/2C2T - Artigos em revistas internacionais com arbitragem científica |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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2012 Hydroxylation of polypropylene using the monooxygenase mutant 139-3 from Bacillus megaterium BM3.pdf | 232,91 kB | Adobe PDF | Ver/Abrir |