Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/2583
Título: | Determination and modeling of yeast viability under stress conditions |
Autor(es): | Coutinho, J. A. P. Coelho, M. A. Z. Ferreira, Eugénio C. Belo, Isabel |
Palavras-chave: | Modeling Digital image processing Cell viability Yeast |
Data: | 2004 |
Editora: | Elsevier 1 |
Citação: | COUTINHO, João A. P. [et al] - Determination and modeling of yeast viability under stress conditions. In ESCAPE : european symposium on computer-aided process engineering, 14, Lisbon, 2004 ; European symposium of the working party on computer-aided process engineering, 37, Lisbon, 2004 – “ESCAPE-14 : European Symposium on Computer Aided Process Engineering ; 37th European symposium of the working party on computer-aided process engineering” [CD-ROM]. Amsterdam [etc.] : Elsevier, 2004. ISBN 0-444-51694-8. ISSN 1570-7946. |
Resumo(s): | In this work the effect of the total pressure and the oxygen concentration in the gas phase on the growth and viability of Saccharomyces cerevisiae is studied. Cell viability was measured staining with methylene blue and assessed using digital image processing. A model that takes into account cell viability was developed and used for the correlation of the measured data. A dependence of the death rate, KD, with the total pressure and the oxygen concentration is proposed. Its behavior indicates that opposing effects between oxygen availability and baric and oxidative stresses are present on the system. It is shown that the proposed model can, with this extra parameter, successfully describe not only the traditional X-P-S evolution but also the ratio of viable cells with time. |
Tipo: | Artigo em ata de conferência |
URI: | https://hdl.handle.net/1822/2583 |
ISBN: | 0-444-51694-8 |
ISSN: | 1570-7946 |
Versão da editora: | http://www.elsevier.com/wps/find/homepage.cws_home |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Artigos em Livros de Atas / Papers in Proceedings |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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4.6.27.ESCAPE14_Viability_Final[1].pdf | 54 kB | Adobe PDF | Ver/Abrir |