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https://hdl.handle.net/1822/2559
Título: | Carbohydrate carbon sources induce loss of flocculation of an ale-brewing yeast strain |
Autor(es): | Soares, Eduardo V. Vroman, A. Mortier, J. Rijsbrack, K. Mota, M. |
Palavras-chave: | Carbon source Flocculation NewFlo phenotype Protein synthesis Saccharomyces cerevisiae |
Data: | Mai-2004 |
Editora: | Blackwell Publishing |
Revista: | Journal of applied microbiology |
Citação: | Soares, E. V., Vroman, A., Mortier, J., Rijsbrack, K., & Mota, M. (2004, May). Carbohydrate carbon sources induce loss of flocculation of an ale-brewing yeast strain. Journal of Applied Microbiology. Wiley. http://doi.org/10.1111/j.1365-2672.2004.02240.x |
Resumo(s): | Aims: To identify the nutrients that can trigger the loss of flocculation under growth conditions in an ale-brewing strain, Saccharomyces cerevisiae NCYC 1195. Methods and Results: Flocculation was evaluated using the method of Soares, EX. and Vroman, A. [Journal of Applied Microbiology (2003) 95, 325]. Yeast growth with metabolizable carbon sources (glucose, fructose, galactose, maltose or sucrose) at 2% (w/v), induced the loss of flocculation in yeast that had previously been allowed to flocculate. The yeast remained flocculent when transferred to a medium containing the required nutrients for yeast growth and a sole nonmetabolizable carbon source (lactose). Transfer of flocculent yeast into a growth medium with ethanol (4% v/v), as the sole carbon source did not induce the loss of flocculation. Even the addition of glucose (2% w/v) or glucose and antimycin A (0.1 mg lˉ¹) to this culture did not bring about loss of flocculation. Cycloheximide addition (15 mglˉ¹) to glucose-growing cells stopped flocculation loss. Conclusions: Carbohydrates were the nutrients responsible for stimulating the loss of flocculation in flocculent yeast cells transferred to growing conditions. The glucose-induced loss of flocculation required de novo protein synthesis. Ethanol prevented glucose-induced loss of flocculation. This protective effect of ethanol was independent of the respiratory function of the yeast. Significance and Impact of the Study: This work contributes to the elucidation of the role of nutrients in the control of the flocculation cycle in NewFlo phenotype yeast strains. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/2559 |
DOI: | 10.1111/j.1365-2672.2004.02240.x |
ISSN: | 1364-5072 |
e-ISSN: | 1365-2672 |
Versão da editora: | https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2004.02240.x |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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2004-42-Soares_etal[1].pdf | 115,29 kB | Adobe PDF | Ver/Abrir |