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TitleAdvances and drawbacks of the adaptation to serum-free culture of CHO-K1 cells for monoclonal antibody production
Author(s)Rodrigues, E.
Costa, A. R.
Henriques, Mariana
Cunnah, Philip
Melton, David
Azeredo, Joana
Oliveira, Rosário
KeywordsChinese hamster ovary cells
Monoclonal antibody
Serum-free medium
Trace elements
Cell adaptation
Issue date2013
JournalApplied Biochemistry and Biotechnology
Abstract(s)Currently, mammalian cell technology has become the focus of biopharmaceutical production, with strict regulatory scrutiny of the techniques employed. Major concerns about the presence of animal-derived components in the culture media led to the development of serum-free (SF) culture processes. However, cell adaptation to SF conditions is still a major challenge and limiting step of process development. Thus, this study aims to assess the impact of SF adaptation on monoclonal antibody production (mAb), identify the most critical steps of cell adaptation to the SF EX-CELL medium, and create basic process guidelines.. The success of SF adaptation was dependent on critical steps that included: accentuated cell sensitivity to common culture procedures (centrifugation, trypsinization); initial cell concentration; time given at each step of serum-reduction; and, most importantly, medium supplements used to support adaptation. Indeed, only one of the five supplement combinations assessed (rhinsulin, ammonium metavanadate, nickel chloride and stannous chloride) succeeded for the CHO-K1 cell line used. This work also revealed that the chemically-defined EX-CELL medium benefits mAb production in comparison with the general purpose Dulbecco's Modified Eagle's Medium, but the complete removal of serum attenuates these positive effects.
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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