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https://hdl.handle.net/1822/25020
Título: | Validation of a fluorescence in situ hybridization method using peptide nucleic acid probes for detection of Helicobacter pylori clarithromycin resistance in gastric biopsy specimens |
Autor(es): | Cerqueira, L. Fernandes, R. M. Ferreira, Rui M. Oleastro, Mónica Carneiro, Fátima Brandão, Catarina Nunes, Pedro Pimentel Ribeiro, Mário Dinis Figueiredo, C. Keevil, C. W. Vieira, M. J. Azevedo, N. F. |
Data: | 2013 |
Editora: | American Society of Microbiology |
Revista: | Journal of Clinical Microbiology |
Resumo(s): | Here, we evaluated a previously established peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) method as a new diagnostic test for Helicobacter pylori clarithromycin resistance detection in paraffin-embedded gastric biopsy specimens. Both a retrospective study and a prospective cohort study were conducted to evaluate the specificity and sensitivity of a PNA-FISH method to determine H. pylori clarithromycin resistance. In the retrospective study (n=30 patients), full agreement between PNA-FISH and PCR-sequencing was observed. Compared to the reference method (culture followed by Etest), the specificity and sensitivity of PNA-FISH were 90.9% (95% confidence interval [CI], 57.1% to 99.5%) and 84.2% (95% CI, 59.5% to 95.8%), respectively. In the prospective cohort (n=93 patients), 21 cases were positive by culture. For the patients harboring clarithromycin- resistant H. pylori, the method showed sensitivity of 80.0% (95% CI, 29.9% to 98.9%) and specificity of 93.8% (95% CI, 67.7% to 99.7%). These values likely represent underestimations, as some of the discrepant results corresponded to patients infected by more than one strain. PNA-FISH appears to be a simple, quick, and accurate method for detecting H. pylori clarithromycin resistance in paraffin-embedded biopsy specimens. It is also the only one of the methods assessed here that allows direct and specific visualization of this microorganism within the biopsy specimens, a characteristic that allowed the observation that cells of different H. pylori strains can subsist in very close proximity in the stomach. Copyright © 2013, American Society for Microbiology. All Rights Reserved. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/25020 |
DOI: | 10.1128/JCM.00302-13 |
ISSN: | 1098-660X |
e-ISSN: | 0095-1137 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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1887.full.pdf | 489,23 kB | Adobe PDF | Ver/Abrir |