Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/24347
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Campo DC | Valor | Idioma |
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dc.contributor.author | Ferreira, José A. | - |
dc.contributor.author | Pires, Cristiana | - |
dc.contributor.author | Paulo, Marina | - |
dc.contributor.author | Azevedo, N. F. | - |
dc.contributor.author | Domingues, M. R. M. | - |
dc.contributor.author | Vieira, M. J. | - |
dc.contributor.author | Monteiro, Mario A. | - |
dc.contributor.author | Coimbra, Manuel A. | - |
dc.date.accessioned | 2013-06-05T17:58:44Z | - |
dc.date.available | 2013-06-05T17:58:44Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 1083-4389 | por |
dc.identifier.uri | https://hdl.handle.net/1822/24347 | - |
dc.description.abstract | Background: Helicobacter pylori cell surface is composed of lipopolysaccharides (LPSs) yielding structures homologous to mammalian Lewis O-chains blood group antigens. These structures are key mediators in the definition of host-microbial interactions and known to change their expression pattern in response to environmental pressure. Aims: The present work is focused on the identification of new H. pylori cell-surface glycosides. Special attention is further devoted to provide insights on the impact of in vitro subcultivation on H. pylori cell-surface phenotypes. Methods: Cell-surface glycans from H. pylori NCTC 11637 and two clinical isolates were recovered from the aqueous phase resulting from phenol:water extraction of intact bacteria. They were evaluated in relation to their sugars and glycosidic-linkages composition by CG-MS, size-exclusion chromatography, NMR, and Mass Spectrometry. H. pylori glycan profile was also monitored during subcultivation in vitro in agar and F12 liquid medium. Results: All three studied strains produce LPS expressing Lewis epitopes and express bioaccumulate amylose-like glycans. Bioaccumulation of amylose was found to be enhanced with the subcultivation of the bacterium on agar medium and accompanied by a decrease in the expression of LPS O-chains. In contrast, during exponential growth in F12 liquid medium, an opposite behavior is observed, that is, there is an increase in the overall amount of LPS and decrease in amylose content. Conclusions: This work shows that under specific environmental conditions, H. pylori expresses a phase-variable cell-surface α-(1→4)-glucose moiety. | por |
dc.language.iso | eng | por |
dc.publisher | Wiley-Blackwell | - |
dc.rights | openAccess | por |
dc.subject | Helicobacter pylori | por |
dc.subject | Cell-surface polysaccharide | por |
dc.subject | Glucan | por |
dc.subject | Environmental pressure | por |
dc.title | Bioaccumulation of amylose-like glycans by Helicobacter pylori | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 559 | por |
oaire.citationEndPage | 570 | por |
oaire.citationIssue | 6 | por |
oaire.citationTitle | Helicobacter | por |
oaire.citationVolume | 14 | por |
dc.publisher.uri | WILEY-Blackwell | por |
dc.identifier.eissn | 1523-5378 | por |
dc.identifier.doi | 10.1111/j.1523-5378.2009.00725.x | por |
dc.identifier.pmid | 19889074 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Helicobacter | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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j.1523-5378.2009.00725.x.pdf | 559,88 kB | Adobe PDF | Ver/Abrir |