Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/24338

TitleThe impact of cell adaptation to serum-free conditions on the glycosylation profile of a monoclonal antibody produced by CHO cells
Author(s)Costa, A. R.
Withers, Joanne
Rodrigues, E.
McLoughlin, Niaobh
Henriques, Mariana
Oliveira, Rosário
Rudd, Pauline M.
Azeredo, Joana
KeywordsMonoclonal antibody
Chinese hamster
Serum-free
Adaptation
Glycosylation
Issue date2013
PublisherElsevier
JournalNew Biotechnology
Abstract(s)N-glycosylation is one of the most critical parameters affecting the biological activity of therapeutic monoclonal antibodies (mAbs), and should therefore be closely monitored and controlled to guarantee a consistent and high-quality product in biopharmaceutical processes. In the present work, the effect of the time-consuming step of gradual cell adaptation to serum-free conditions on the glycosylation profile of a mAb produced by CHO-K1 cells was evaluated. High-performance liquid chromatography analysis revealed important changes in mAb glycosylation patterns in all steps of serum reduction. These changes could be grouped in two distinct phases of the process of adaptation: middle (2.5 to 0.15% serum) and final (0.075 and 0% serum). For intermediate levels of serum, a desirable increase of galactosylation and decrease of fucosylation, but an undesirable increase in sialylation were observed; while the inverse was obtained at the final stages of adaptation. These divergences may be related to the reduction of serum supplementation, to variations in the levels of cell density and viability achieved at these stages, and to the natural shift of the cell growth mode during adaptation from adherent to suspended. The divergent glycan profiles obtained in this study demonstrate a strong influence of the adaptation process on mAb glycosylation, suggesting that control and monitoring of product quality should be implemented at the early stages of process development.
Typearticle
URIhttp://hdl.handle.net/1822/24338
DOI10.1016/j.nbt.2012.12.002
ISSN1871-6784
e-ISSN1871-6784
Peer-Reviewedyes
AccessrestrictedAccess
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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