Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/24081

TitleDevelopment of macro/micro porous silk fibroin scaffolds with nano-sized calcium phosphate particles for bone tissue engineering
Author(s)Yan, Leping
Correia, Cristina
Silva-Correia, Joana
Caridade, S. G.
Fernandes, E. M.
Mano, J. F.
Sousa, R. A.
Oliveira, Joaquim M.
Oliveira, A. L.
Reis, R. L.
KeywordsBone
Silk fibroin
Issue dateSep-2012
PublisherJohn Wiley and Sons
JournalJournal of Tissue Engineering and Regenerative Medicine
CitationYan L. - P. Yan L. P., Correia C., Silva-Correia J., Caridade S. G., Fernandes E. M., Mano J. F., Sousa R. A., Oliveira J. M., Oliveira A. L., Reis R. L. Preparation and characterization of macro/micro porous silk fibroin /nano-sized calcium phosphate scaffolds for bone tissue engineering, Journal of tissue engineering and regenerative medicine, Vol. 6, Issue Suppl.1, pp. 181, doi:10.1002/term.1586, 2012
Abstract(s)Macro/micro porous silk scaffolds with nano-sized calcium phosphate (CaP) particles were developed for bone tissue engineering. Different amounts of nano-sized CaP particles 4, 8, 16 and 25% (CaP:silk fibroin, wt/wt) were generated into the highly concentrated aqueous silk fibroin solutions via an in-situ approach. Afterwards, the pure silk and silk/nano-CaP porous scaffolds were produced by a combination of salt-leaching/freeze-drying methods. Thermal gravimetric analysis results were able to demonstrated that the silk/nano-CaP scaffolds maintained 64–87% of incorporated CaP after salt-leaching. Dynamic mechanical analysis showed that storage modulus of the 16% formulation was significantly higher than all remaining groups. The porosity of silk/nano-CaP scaffolds assessed using Micro-Computed Tomography decreased from 79.8% to 63.6% with increasing CaP incorporated until 16%. By soaking the scaffolds in Simulated Body Fluid for 7 days, cauliflower-like apatite clusters were observed on the surface of both macro and micro pores of 16% and 25% formulations, which was not observed in 4 and 8% formulations. 16% silk/nano-CaP scaffolds were further chosen for in vitro cytotoxicity and biocompatibility assays. Both silk and 16% silk/nano-CaP scaffolds were non-cytotoxic and promoted cell adhesion and proliferation to a similar extent. These results indicated that the 16% silk/ nano-CaP scaffolds could be a good candidate for bone tissue engineering.
TypeAbstract
URIhttp://hdl.handle.net/1822/24081
ISSN1932-6254
Peer-Reviewedyes
AccessOpen access
Appears in Collections:3B’s - Resumos em livros de atas de conferências - indexados no ISI Web of Science

Files in This Item:
File Description SizeFormat 
15891-1349027738_Abstract of TERM 2012.Vienna 181.pdf60,02 kBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID