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https://hdl.handle.net/1822/23857
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Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Zonari, A. A. C. | - |
dc.contributor.author | Cerqueira, M. T. | - |
dc.contributor.author | Novikoff, S. | - |
dc.contributor.author | Goes, A. M. | - |
dc.contributor.author | Marques, A. P. | - |
dc.contributor.author | Correlo, V. M. | - |
dc.contributor.author | Reis, R. L. | - |
dc.date.accessioned | 2013-04-30T10:05:27Z | - |
dc.date.available | 2013-04-30T10:05:27Z | - |
dc.date.issued | 2012-09 | - |
dc.date.submitted | 2012-09 | - |
dc.identifier.issn | 1932-6254 | por |
dc.identifier.uri | https://hdl.handle.net/1822/23857 | - |
dc.description.abstract | Polyhydroxybutyrate-co-hydroxyvalerate (PHB-HV) is natural-based polyester produced by microorganisms under unbalanced growth con- ditions. This polymer is known for its biocompatibility and biodegrad- ability and has been explored for tissue engineering approaches. The aim of this work was to create a bi-layered construct, based on PHB- HV, for skin tissue replacement. The PHB-HV bi-layered scaffold was produced taking into consideration the two main skin strata, the epi- dermis and the dermis. For the epidermal fraction, a membrane was developed using a solvent cast approach, while freeze-drying was used to obtain the dermal fraction. The two structures were combined to obtain a scaffold that has a thin compact surface (epidermal) and a por- ous structure (dermis), as confirmed by scanning electron microscopy (SEM) and micro-CT analysis. Each layer of the scaffold, epidermal and dermal, was respectively seeded with human keratinocytes (Kc) and human dermal fibroblasts (Fb). The characterization by SEM con- firmed the typical morphology of Kc and Fb on the expected surfaces. Calcein-AM and DNA quantification showed that both type of cells remained viable along the experiment and were able to attach and pro- liferate on the respective seeding layers. In conclusion, the developed PHB-HV scaffolds have shown suitable properties to sustain the co-cul- ture of Kc and Fb, which constitutes a solid base for further work to demonstrate its potential for skin replacement. | por |
dc.language.iso | eng | por |
dc.publisher | John Wiley and Sons | por |
dc.rights | openAccess | por |
dc.subject | Natural-based polyester | por |
dc.subject | Polyhydroxybutyrate-co-hydroxyvalerate | por |
dc.title | Evaluation of the potential of polyhydroxybutyrate-co-hydroxyvalerate bi-layered scaffolds for skin tissue engineering | por |
dc.type | conferenceAbstract | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://onlinelibrary.wiley.com/doi/10.1002/term.1586/abstract | por |
dc.comments | http://www.3bs.uminho.pt/node/17257 | - |
sdum.publicationstatus | published | por |
oaire.citationConferenceDate | 2012 | por |
sdum.event.type | conference | por |
oaire.citationStartPage | 97 | por |
oaire.citationEndPage | 97 | por |
oaire.citationIssue | Sup. 1 | - |
oaire.citationConferencePlace | Vienna, Austria | por |
oaire.citationTitle | Journal of Tissue Engineering and Regenerative Medicine (3rd TERMIS World Congress) | por |
oaire.citationVolume | 6 | - |
dc.date.updated | 2013-04-10T14:18:44Z | - |
dc.subject.wos | Science & Technology | por |
sdum.journal | Journal of Tissue Engineering and Regenerative Medicine | por |
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Ficheiro | Descrição | Tamanho | Formato | |
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17257-term.1586-zanori.pdf | 44,04 kB | Adobe PDF | Ver/Abrir |