Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/23596

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dc.contributor.authorCustódio, Catarina A.-
dc.contributor.authorFrias, A. M.-
dc.contributor.authorCampo, Aranzazu del-
dc.contributor.authorReis, R. L.-
dc.contributor.authorMano, J. F.-
dc.date.accessioned2013-04-08T14:30:20Z-
dc.date.available2013-04-08T14:30:20Z-
dc.date.issued2012-11-
dc.date.submitted2012-11-
dc.identifier.issn1934-8630por
dc.identifier.urihttp://hdl.handle.net/1822/23596-
dc.description.abstractBioactive constructs to guide cellular mobilization and function have been proposed as an approach for a new generation of biomaterials in functional tissue engineering. Adult mesenchymal stem cells have been widely used as a source for cell based therapeutic strategies, namely tissue engineering. This is a heterogeneous cell population containing many subpopulations with distinct regenerative capacity. Thus, one of the issues for the effective clinical use of stem cells in tissue engineering is the isolation of a highly purified, expandable specific subpopulation of stem cells. Antibody functionalized biomaterials could be promising candidates to isolate and recruit specific cell types. Here we propose a new concept of instructive biomaterials that are able to recruit and purify specific cell types from a mixed cell population. This biomimetic concept uses a target-specific chitosan substrate to capture specific adipose derived stem cells. Specific antibodies were covalently immobilized onto chitosan membranes using bis[sulfosuccinimidyl] suberate (BS3). Quartz crystal microbalance (QCM) was used to monitor antibody immobilization/adsorption onto the chitosan films. Specific antibodies covalently immobilized kept their bioactivity and captured specific cell types from a mixed cell population. Microcontact printing allowed to covalently immobilize antibodies in patterns and simultaneously a spatial control in cell attachmentpor
dc.description.sponsorshipThe authors acknowledge the Portuguese Foundation for Science and Technology (FCT) for the fellowships SFRH/BD/61390/2009 (C.A.C) and SFRH/BPD/45206/2008 (A.M.F), and also to the International Max-Planck-Research School (C.A.C) for the financial support. We are grateful to Hospital da Prelada for the donations. This work was carried out under the scope of the EU 7th Framework Programme (FP7/2007-2013) under grant agreement no. NMP4-SL-2009-229292 (Find&Bind).por
dc.language.isoengpor
dc.publisherSpringer por
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/229292-
dc.rightsopenAccesspor
dc.subjectBiomolecules immobilizationpor
dc.subjectCell recruitmentpor
dc.subjectInstructive surfacespor
dc.titleSelective cell recruitment and spatially controlled cell attachment on instructive chitosan surfaces functionalized with antibodiespor
dc.typearticle-
dc.peerreviewedyespor
dc.commentshttp://www.3bs.uminho.pt/node/15918por
sdum.publicationstatuspublishedpor
oaire.citationStartPage2por
oaire.citationEndPage9por
oaire.citationIssue65por
oaire.citationTitleBiointerphasespor
oaire.citationVolume7por
dc.date.updated2013-04-08T11:53:01Z-
dc.identifier.doi10.1007/s13758-012-0065-3por
dc.identifier.pmid23109106por
dc.subject.wosScience & Technologypor
sdum.journalBiointerphasespor
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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