Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/23399

TitleProposal for a method to estimate nutrient shock effects in bacteria
Author(s)Azevedo, N. F.
Bragança, S. M.
Simões, Lúcia C.
Cerqueira, L.
Almeida, Carina
Keevil, C. W.
Vieira, M. J.
KeywordsNutrient shock
Osmotic shock
Nutrient stress substrate-accelerated death
Substrate-accelerated death
Nutrient stress
Issue date2012
JournalBMC Research Notes
Abstract(s)Plating methods are still the golden standard in microbiology; however, some studies have shown that these techniques can underestimate the microbial concentrations and diversity. A nutrient shock is one of the mechanisms proposed to explain this phenomenon. In this study, a tentative method to assess nutrient shock effects was tested. Findings To estimate the extent of nutrient shock effects, two strains isolated from tap water (Sphingomonas capsulata and Methylobacterium sp.) and two culture collection strains (E. coli CECT 434 and Pseudomonas fluorescens ATCC 13525) were exposed both to low and high nutrient conditions for different times and then placed in low nutrient medium (R2A) and rich nutrient medium (TSA). The average improvement (A.I.) of recovery between R2A and TSA for the different times was calculated to more simply assess the difference obtained in culturability between each medium. As expected, A.I. was higher when cells were plated after the exposition to water than when they were recovered from high-nutrient medium showing the existence of a nutrient shock for the diverse bacteria used. S. capsulata was the species most affected by this phenomenon. Conclusions This work provides a method to consistently determine the extent of nutrient shock effects on different microorganisms and hence quantify the ability of each species to deal with sudden increases in substrate concentration.
TypeArticle
URIhttp://hdl.handle.net/1822/23399
DOI10.1186/1756-0500-5-422
ISSN1756-0500
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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