Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/23149
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Campo DC | Valor | Idioma |
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dc.contributor.author | Martins, Ana M. | - |
dc.contributor.author | Kretlow, J. D. | - |
dc.contributor.author | Pinto, A. R. | - |
dc.contributor.author | Malafaya, P. B. | - |
dc.contributor.author | Fernandes, E. M. | - |
dc.contributor.author | Neves, N. M. | - |
dc.contributor.author | Alves, Catarina M. | - |
dc.contributor.author | Mikos, Antonios G. | - |
dc.contributor.author | Kasper, F. Kurtis | - |
dc.contributor.author | Reis, R. L. | - |
dc.date.accessioned | 2013-02-20T16:29:27Z | - |
dc.date.available | 2013-02-20T16:29:27Z | - |
dc.date.issued | 2012-02 | - |
dc.date.submitted | 2012-02 | - |
dc.identifier.issn | 1552-4965 | por |
dc.identifier.uri | https://hdl.handle.net/1822/23149 | - |
dc.description.abstract | This study used a rat subcutaneous implantation model to investigate gradual in situ pore formation in a self-regulating degradable chitosan-based material, which comprises lysozyme incorporated into biomimetic calcium phosphate (CaP) coatings at the surface to control the scaffold degradation and subsequent pore formation. Specifically, the in vivo degradation of the scaffolds, the in situ pore formation, and the tissue response were investigated. Chitosan or chitosan/starch scaffolds were studied with and without a CaP coating in the presence or absence of lysozyme for a total of six experimental groups. Twenty-four scaffolds per group were implanted, and eight scaffolds were retrieved at each of three time points (3, 6, and 12 weeks). Harvested samples were analyzed for weight loss, microcomputed tomography, and histological analysis. All scaffolds showed pronounced weight loss and pore formation as a function of time. The highest weight loss was 29.8% 6 1.5%, obtained at week 12 for CaP chitosan/starch scaffolds with lysozyme incorporated. Moreover, all experimental groups showed a significant increase in porosity after 12 weeks. At all time points no adverse tissue reaction was observed, and as degradation increased, histological analysis showed cellular ingrowth throughout the implants. Using this innovative methodology, the ability to gradually generate pores in situ was clearly demonstrated in vivo. | por |
dc.description.sponsorship | Contract grant sponsor: Portuguese Foundation for Science and Technology (FCT); contract grant numbers: SFRH/BPD/66897/2009, SFRH/BPD/26763/2006, SFRH/BD/24735/2005Contract grant sponsor: European NoE EXPERTISSUES; contract grant number: NMP3-CT-2004-500283Contract grant sponsor: National Institutes of Health; contract grant number: R01 DE17441Contract grant sponsor: Baylor College of Medicine Medical Scientist Training Program; contract grant number: NIH T32 GM07330Contract grant sponsor: Rice Institute of Biosciences and Bioengineering's Biotechnology Training Grant; contract grant number: NIH T32 GM008362Contract grant sponsor: Keck Center Nanobiology Training Program of the Gulf Coast Consortia; contract grant number: 5 T90 DK070121-04 | por |
dc.language.iso | eng | por |
dc.publisher | John Wiley and Sons | por |
dc.rights | openAccess | por |
dc.subject | Enzymes | por |
dc.subject | In situ pore formation | por |
dc.subject | In vivo degradation | por |
dc.subject | Natural origin materials | por |
dc.subject | Tissue response | por |
dc.title | Gradual pore formation in natural origin scaffolds throughout subcutaneous implantation | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.comments | http://www.3bs.uminho.pt/node/15816 | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 599 | por |
oaire.citationEndPage | 612 | por |
oaire.citationIssue | 3 | por |
oaire.citationVolume | 100 | por |
dc.date.updated | 2013-02-19T18:07:29Z | - |
dc.identifier.doi | 10.1002/jbm.a.33261 | por |
dc.identifier.pmid | 22213676 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Journal of Biomedical Materials Research: Part A | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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1327078857_AMMartins_JBMR_A_33261_fta.pdf | 1,99 MB | Adobe PDF | Ver/Abrir |