Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/22306

TitleUnraveling the uptake mechanisms of mannan nanogel in bone-marrow-derived macrophages
Author(s)Ferreira, Sílvia A.
Correia, Alexandra
Madureira, Pedro
Vilanova, Manuel
Gama, F. M.
KeywordsNanogel
Cell internalization
Confocal microscopy
NanogelCell
endocytosis
imaging
mannan nanogel
self-assembly
Issue date2012
PublisherJohn Wiley and Sons
JournalMacromolecular Bioscience
Abstract(s)The mechanisms associated with the cellular internalization of nanomedicines must be carefully considered when designing drug- and vaccine-delivery systems. The cellular fate and effects of nanomedicines depend to a large extent on the cell uptake routes. A self-assembled mannan nanogel is developed as a vaccination platform for antigen and adjuvant delivery. The mannan nanogel uptake by murine bone-marrow-derived macrophages is found to be time-, concentration-, and energy-dependent, involving mannose-receptor-mediated phagocytosis and clathrin-mediated endocytosis. The nanogel is also visualized in the cytosol suggesting endolysosomal escape. These results indicate that mannan nanogel is a promising versatile carrier for intracellular delivery of vaccines or therapeutic agents.
TypeArticle
URIhttp://hdl.handle.net/1822/22306
DOI10.1002/mabi.201200075
ISSN1616-5187
Other identifiers1616-5195
Peer-Reviewedno
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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