Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/22306
Título: | Unraveling the uptake mechanisms of mannan nanogel in bone-marrow-derived macrophages |
Autor(es): | Ferreira, Sílvia A. Correia, Alexandra Madureira, Pedro Vilanova, Manuel Gama, F. M. |
Palavras-chave: | Nanogel Cell internalization Confocal microscopy NanogelCell endocytosis imaging mannan nanogel self-assembly |
Data: | 2012 |
Editora: | John Wiley and Sons |
Revista: | Macromolecular Bioscience |
Resumo(s): | The mechanisms associated with the cellular internalization of nanomedicines must be carefully considered when designing drug- and vaccine-delivery systems. The cellular fate and effects of nanomedicines depend to a large extent on the cell uptake routes. A self-assembled mannan nanogel is developed as a vaccination platform for antigen and adjuvant delivery. The mannan nanogel uptake by murine bone-marrow-derived macrophages is found to be time-, concentration-, and energy-dependent, involving mannose-receptor-mediated phagocytosis and clathrin-mediated endocytosis. The nanogel is also visualized in the cytosol suggesting endolysosomal escape. These results indicate that mannan nanogel is a promising versatile carrier for intracellular delivery of vaccines or therapeutic agents. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/22306 |
DOI: | 10.1002/mabi.201200075 |
ISSN: | 1616-5187 |
Outros identificadores: | 1616-5195 |
Arbitragem científica: | no |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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1172_ftp.pdf | 591,55 kB | Adobe PDF | Ver/Abrir |