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TitleUnraveling the uptake mechanisms of mannan nanogel in bone-marrow-derived macrophages
Author(s)Ferreira, Sílvia A.
Correia, Alexandra
Madureira, Pedro
Vilanova, Manuel
Gama, F. M.
Cell internalization
Confocal microscopy
mannan nanogel
Issue date2012
PublisherJohn Wiley and Sons
JournalMacromolecular Bioscience
Abstract(s)The mechanisms associated with the cellular internalization of nanomedicines must be carefully considered when designing drug- and vaccine-delivery systems. The cellular fate and effects of nanomedicines depend to a large extent on the cell uptake routes. A self-assembled mannan nanogel is developed as a vaccination platform for antigen and adjuvant delivery. The mannan nanogel uptake by murine bone-marrow-derived macrophages is found to be time-, concentration-, and energy-dependent, involving mannose-receptor-mediated phagocytosis and clathrin-mediated endocytosis. The nanogel is also visualized in the cytosol suggesting endolysosomal escape. These results indicate that mannan nanogel is a promising versatile carrier for intracellular delivery of vaccines or therapeutic agents.
Other identifiers1616-5195
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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