Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/20648
Registo completo
Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Salgado, A. J. | - |
dc.contributor.author | Oliveira, Joaquim M. | - |
dc.contributor.author | Pirraco, Rogério | - |
dc.contributor.author | Pereira, Vitor H. | - |
dc.contributor.author | Fraga, J. S. | - |
dc.contributor.author | Marques, A. P. | - |
dc.contributor.author | Neves, N. M. | - |
dc.contributor.author | Mano, J. F. | - |
dc.contributor.author | Reis, R. L. | - |
dc.contributor.author | Sousa, Nuno | - |
dc.date.accessioned | 2012-11-02T18:08:17Z | - |
dc.date.available | 2012-11-02T18:08:17Z | - |
dc.date.issued | 2010-10 | - |
dc.identifier.issn | 1616-5187 | por |
dc.identifier.uri | https://hdl.handle.net/1822/20648 | - |
dc.description.abstract | The applicability of CMCht/PAMAM dendrimer nanoparticles for CNS applications was investigated. AFM and TEM observations revealed that the nanoparticles possessed a nanosphere- like shape with a size from 22.0 to 30.7 nm. The nanoparticles could be bound to fluorescent-probe FITC for tracing purposes. Post-natal hippocampal neurons and cortical glial cells were both able to internalize the FITC-labeled CMCht/PAMAM dendrimer nanoparticles with high efficiency. The percentage of positive cells internalizing the nanoparticles varied, reaching a peak after 48 h of incubation. Further experiments for periods up to 7 d revealed that the periodical addition of FITC-labelled CMCht/ PAMAM dendrimer nanoparticles was needed to maintain the overall percentage of cells internalizing them. Finally, it was also observed that cell viability was not significantly affected by the incubation of dendrimer nanoparticles. | por |
dc.description.sponsorship | Financial support from the Portuguese Foundation for Science and Technology through funds from POCTI and/or FEDER programs (Funding to ICVS, 3B's Research Group, post-doctoral and pre-doctoral fellowships to A.J. Salgado and J.M. Oliveira - SFRH/BPD/17595/2004; SFRH/BD/21786/2005) is gratefully acknowledged. This work was also carried out under the scope of the European NoE EXPERTISSUES (NMP3-CT-2004-500283) and HIPPOCRATES STREP (NMP3-CT-2003-505758) projects. | por |
dc.language.iso | eng | por |
dc.publisher | Wiley-VCH Verlag | por |
dc.rights | openAccess | por |
dc.subject | Cell viability | por |
dc.subject | Dendrimers | por |
dc.subject | Glial cells | por |
dc.subject | Internalization | por |
dc.subject | Intracellular drug delivery systems | por |
dc.title | Carboxymethylchitosan/poly (amidoamine) dendrimer nanoparticles in central nervous systems-regenerative medicine: effects on neuron/glial cell viability and internalization efficiency | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://onlinelibrary.wiley.com/doi/10.1002/mabi.201000005/pdf | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 1130 | por |
oaire.citationEndPage | 1140 | por |
oaire.citationIssue | 10 | por |
oaire.citationTitle | Macromolecular Bioscience | por |
oaire.citationVolume | 10 | por |
dc.identifier.doi | 10.1002/mabi.201000005 | por |
dc.identifier.pmid | 20602413 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Macromolecular Bioscience | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals ICVS - Artigos em revistas internacionais / Papers in international journals |