Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/20638

TitleEffects of starch/polycaprolactone-based blends for spinal cord injury regeneration in neurons/glial cells viability and proliferation
Author(s)Salgado, A. J.
Sousa, R. A.
Fraga, J. S.
Pêgo, José M.
Silva, B. A.
Malva, J. O.
Neves, N. M.
Reis, R. L.
Sousa, Nuno
KeywordsBiodegrable polymers
Starch-poly(ε-caprolactone) blends
Cytotoxicity
Neurons
Glial
Spinal cord injury
Tissue engineering
starch-poly(epsilon-caprolactone) blends
Starch-poly(μ-caprolactone) blends
Issue date2009
PublisherSAGE
JournalJournal of Bioactive and Compatible Polymers
Abstract(s)Spinal cord injury (SCI) leads to drastic alterations on the quality of life of afflicted individuals. With the advent of Tissue Engineering and Regenerative Medicine where approaches combining biomaterials, cells and growth factors are used, one can envisage novel strategies that can adequately tackle this problem. The objective of this study was to evaluate a blend of starch with poly(ε-caprolactone) (SPCL) aimed to be used for the development of scaffolds spinal cord injury (SCI) repair. SPCL linear parallel filaments were deposited on polystyrene coverslips and assays were carried out using primary cultures of hippocampal neurons and glial cells. Light and fluorescence microscopy observations revealed that both cell populations were not negatively affected by the SPCL-based biomaterial. MTS and total protein quantification indicated that both cell viability and proliferation rates were similar to controls. Both neurons and astrocytes occasionally contacted the surface of SPCL filaments through their dendrites and cytoplasmatic processes, respectively, while microglial cells were unable to do so. Using single cell [Ca2+ ]i imaging, hippocampal neurons were observed growing within the patterned channels and were functional as assessed by the response to a 30 mM KCl stimulus. The present data demonstrated that SPCL-based blends are potentially suitable for the development of scaffolds in SCI regenerative medicine.
TypeArticle
URIhttp://hdl.handle.net/1822/20638
DOI10.1177/0883911509104081
ISSN0883-9115
Publisher versionhttp://jbc.sagepub.com/cgi/content/abstract/24/3/235
Peer-Reviewedyes
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

Files in This Item:
File Description SizeFormat 
file.pdf312,05 kBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID