Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/20566

TitleSurface modification of a biodegradable composite by UV laser ablation : in vitro biological performance
Author(s)Martins, Albino
Gang, Wu
Pinho, Elisabete D.
Rebollar, Esther
Chiussi, Stefano
Reis, R. L.
Leon, Betty
Neves, N. M.
KeywordsSurface modification
Biodegradable composite
Laser ablation
Surface properties
Biological performance
Issue dateJan-2010
PublisherWiley
JournalJournal of Tissue Engineering and Regenerative Medicine
Abstract(s)Melt blends of chitosan and biodegradable aliphatic polyester have been physically and biologically studied, presenting great potential for biomedical applications. Structurally, poly(butylene succinate)–chitosan (PBS/Cht) composite scaffolds are covered by a thin PBS layer, preventing the desired interaction of cells/tissues with the chitosan particules. In the present work, a selective and controlled ablation of this skin layer was induced by UV laser processing. X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF–SIMS) data demonstrated an increment of chitosan components and others resulting from the laser ablation process. The biological activity (i.e. cell viability and proliferation) on the inner regions of the composite scaffolds is not significantly different from those of the external layer, despite the observed differences in surface roughness (determined by interferometric optical profilometry) and wettability (water contact angle). However, the morphology of human osteoblastic cells was found to be considerably different in the case of laser-processed samples, since the cells tend to aggregate in multilayer columnar structures, preferring the PBS surface and avoiding the chitosan-rich areas. Thus, UV laser ablation can be considered a model technique for the physical surface modification of biomaterials without detrimental effects on cellular activity.
TypeArticle
URIhttp://hdl.handle.net/1822/20566
DOI10.1002/term.255
ISSN1932-6254
Peer-Reviewedyes
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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