Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/20545

TitleEnzymatic degradation of 3D scaffolds of starch-poly-(epsilon-caprolactone) prepared by supercritical fluid technology
Author(s)Duarte, Ana Rita C.
Mano, J. F.
Reis, R. L.
KeywordsSupercritical fluids
Starch
Polycaprolactone
Natural polymers
Enzymatic degradation
Tissue engineering
Issue date2010
PublisherElsevier
JournalPolymer Degradation and Stability
Abstract(s)Starch-based polymers have been proposed for different tissue engineering applications due to their inherent properties. In this work, a polymeric blend of starch-poly-(3-caprolactone) (SPCL) was processed using supercritical fluid technology, namely, by supercritical assisted phase inversion. As SPCL is a biodegradable polymer, the matrices produced are susceptible of undergoing enzymatic degradation upon implantation in the human body. In vitro assessment of the enzymatic degradation of SPCL was carried out in different buffer solutions containing a-amylase and/or lipase. The effect of the presence of these enzymes was studied by monitoring different parameters in order to characterise both bulk and the surface of the scaffolds. As regards to bulk analysis, weight loss of the samples incubated for 1, 3, 7, 14 and 21 days was determined, further differential scanning calorimetry was carried out. The morphology of the scaffolds after these periods was analysed by micro-computed tomography (m-CT) and surface chemistry was characterised by infra-red spectroscopy and contact angle measurements. Results suggest that SPLC scaffolds undergo bulk degradation, which is typically characterised by hydrolysis of chemical bonds in the polymer chain at the centre of the matrix, resulting in a highly porous material.
TypeArticle
URIhttp://hdl.handle.net/1822/20545
DOI10.1016/j.polymdegradstab.2010.06.020
ISSN0141-3910
Publisher versionhttp://www.sciencedirect.com/
Peer-Reviewedyes
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

Files in This Item:
File Description SizeFormat 
file.pdf819,49 kBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID