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|Title:||Silk fibroin microparticles as carriers for delivery of human recombinant BMPs: physical characterization and drug release|
|Author(s):||Bessa, Paulo C.|
Balmayor, Elizabeth Rosado
Azevedo, Helena S.
van Griensven, M.
Reis, R. L.
|Keywords:||Bone morphogenetic protein|
|Journal:||Journal of Tissue Engineering and Regenerative Medicine|
|Abstract(s):||Bone morphogenetic proteins (BMPs) are cytokines with strong ability to promote new bone formation. Herein, we report the use of silk fibroin microparticles as carriers for the delivery of BMP-2, BMP-9 or BMP-14. BMP-containing fibroin microparticles were prepared by a mild methodology using dropwise addition of ethanol, exhibiting mean diameters of 2.7 ± 0.3 μm. Encapsulation efficiencies varied between 67.9 ± 6.1 % and 97.7 ± 2.0 % depending on the type and the amount of BMP loaded. Release kinetics showed that BMP-2, BMP-9 and BMP-14 were released in two phases profile, with a burst release in the first two days followed by a slower release, for a period of 14 days. The release data were best explained by Korsmeyer’s model and the Fickian model of drug diffusion. Silk fibroin microparticles can offer a promising approach for the sustained delivery of different BMPs in tissue engineering applications.|
|Appears in Collections:||3B’s - Artigos em revistas/Papers in scientific journals|