Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/20171

TítuloComparison of commercial serum-free media for CHO-K1 cell growth and monoclonal antibody production
Autor(es)Rodrigues, E.
Costa, A. R.
Henriques, Mariana
Azeredo, Joana
Oliveira, Rosário
Palavras-chaveSerum-free media
CHO cells
Monoclonal antibody
Production
Cell growth
Data9-Ago-2012
EditoraElsevier
RevistaInternational Journal of Pharmaceutics
Resumo(s)The selection of a serum-free medium for a particular process of production using mammalian cells is a critical step for its success. In this study, seven commercially available serum-free media (EX-CELL, ISF-I, CD CHO, CDM4CHO, CHO-III-A, Octomed and HybridoMed) were evaluated and compared for cell growth and monoclonal antibody (mAb) production of a transfected CHO-K1 cell line. In the conditions assayed, EX-CELL and particularly CDM4CHO are the most recommended media for extended biopharmaceutical processes, on account of inducing superior levels of cell proliferation and mAb production, accentuated by a tendency to improve over time. Furthermore, the less positive results obtained with some media emphasize the importance and the impact of the correct medium selection.
Tipoarticle
URIhttp://hdl.handle.net/1822/20171
DOI10.1016/j.ijpharm.2012.08.002
ISSN0378-5173
Versão da editorahttp://dx.doi.org/10.1016/j.ijpharm.2012.08.002
Arbitragem científicayes
AcessorestrictedAccess
Aparece nas coleções:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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