Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/20171
Título: | Comparison of commercial serum-free media for CHO-K1 cell growth and monoclonal antibody production |
Autor(es): | Rodrigues, E. Costa, A. R. Henriques, Mariana Azeredo, Joana Oliveira, Rosário |
Palavras-chave: | Serum-free media CHO cells Monoclonal antibody Production Cell growth |
Data: | 9-Ago-2012 |
Editora: | Elsevier 1 |
Revista: | International Journal of Pharmaceutics |
Resumo(s): | The selection of a serum-free medium for a particular process of production using mammalian cells is a critical step for its success. In this study, seven commercially available serum-free media (EX-CELL, ISF-I, CD CHO, CDM4CHO, CHO-III-A, Octomed and HybridoMed) were evaluated and compared for cell growth and monoclonal antibody (mAb) production of a transfected CHO-K1 cell line. In the conditions assayed, EX-CELL and particularly CDM4CHO are the most recommended media for extended biopharmaceutical processes, on account of inducing superior levels of cell proliferation and mAb production, accentuated by a tendency to improve over time. Furthermore, the less positive results obtained with some media emphasize the importance and the impact of the correct medium selection. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/20171 |
DOI: | 10.1016/j.ijpharm.2012.08.002 |
ISSN: | 0378-5173 |
Versão da editora: | http://dx.doi.org/10.1016/j.ijpharm.2012.08.002 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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1-s2.0-S037851731200796X-main.pdf Acesso restrito! | Documento principal | 214,78 kB | Adobe PDF | Ver/Abrir |