Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/1962
Registo completo
Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Cao, Xue-jun | - |
dc.contributor.author | Wu, Xing-yan | - |
dc.contributor.author | Fonseca, Luís Pina | - |
dc.contributor.author | Cabral, J. M. S. | - |
dc.contributor.author | Marcos, João Carlos | - |
dc.date.accessioned | 2005-06-03T13:15:43Z | - |
dc.date.available | 2005-06-03T13:15:43Z | - |
dc.date.issued | 2004 | - |
dc.identifier.citation | "Biotechnology letters". 26 (2004) 97-101. | eng |
dc.identifier.issn | 0141-5492 | por |
dc.identifier.uri | https://hdl.handle.net/1822/1962 | - |
dc.description.abstract | Bioconversion of Penicillin G in PEG 20000-Dextran T 70 aqueous two-phase systems was achieved using the recombinant Escherichia coli A56 (ppA22) with intracellular penicillin acylase as catalyst. The best conversion conditions were attained for: 7%(w/v) substrate (penicillin G), enzyme activity in bottom phase 52 U/ml, pH 7.8, temperature 37°C, reaction time 40 min. Five repeated batches could be performed in these conditions. Conversions ratios between 0.902-0.985mol of 6-aminopenicillanic acid (6-APA) per mol of penicillin G, were obtained and specific productivity was 3.6-4.6 μmol/min•ml. In addition the product 6-APA could directly be crystallized from the top phase with a purity of 96.2%. | eng |
dc.description.sponsorship | Science & Technological Commission of Shanghai Municipal People’s Government. | por |
dc.language.iso | eng | eng |
dc.publisher | Kluwer | eng |
dc.rights | openAccess | eng |
dc.subject | 6-aminopenicillanic acid production | eng |
dc.subject | Aqueous two-phase systems | eng |
dc.subject | Bioconversion | eng |
dc.subject | Recombinant E. coli A56 | eng |
dc.subject | Penicillin acylase | eng |
dc.title | Production of 6-aminopenicillanic acid in aqueous two-phase systems by recombinant Escherichia coli with intracellular penicillin acylase | eng |
dc.type | article | eng |
dc.peerreviewed | yes | eng |
oaire.citationStartPage | 97 | por |
oaire.citationEndPage | 101 | por |
oaire.citationIssue | 2 | por |
oaire.citationVolume | 26 | por |
dc.identifier.doi | 10.1023/B:BILE.0000012885.62462.f3 | por |
dc.identifier.pmid | 15000474 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Biotechnology Letters | por |
Aparece nas coleções: | CDQuim - Artigos (Papers) |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
ArtPenProd_RepUM.pdf | 157,68 kB | Adobe PDF | Ver/Abrir |