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TitleVoltammetric monitoring of laccase-catalysed mediated reactions
Author(s)Fernández-Sánchez, César
Tzanov, Tzanko
Gübitz, Georg M.
Paulo, Artur Cavaco
cyclic voltammetry
catalytic current
Issue date2002
Citation"Bioelectrochemistry". ISSN 1567-5394. 58 (2002) 149-156.
Abstract(s)Six different compounds capable of mediating laccase-catalysed reactions have been tested by cyclic voltammetry. They exhibited quasi-reversible electrodic behaviour with formal redox potentials ranging from 150 to 800 mV (E-0t vs. SCE). The immersion of a laccase-coated glassy carbon electrode (GCE) in mediator solutions generated large cathodic catalytic currents easily recorded by cyclic voltammetry at low-potential scan rates. This current showed two well-defined pH profiles, which correlated with the variation of the mediator redox potentials at the pH range tested. The relevant effect of temperature on the activity of laccase has been assessed here. Likewise, it was shown that the cut-rent record varied with the substrate concentration. This trend fitted Michaelis-Menten kinetics, which allowed us to give an estimation of the affinity of the fungal laccase for the different mediators. (C) 2002 Elsevier Science B.V. All rights reserved.
AccessOpen access
Appears in Collections:DET/2C2T - Artigos em revistas internacionais com arbitragem científica

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