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https://hdl.handle.net/1822/19389
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Campo DC | Valor | Idioma |
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dc.contributor.author | Costa, C. | - |
dc.contributor.author | Alves, A. | - |
dc.contributor.author | Pinto, P. | - |
dc.contributor.author | Sousa, R. A. | - |
dc.contributor.author | Silva, E. | - |
dc.contributor.author | Reis, R. L. | - |
dc.contributor.author | Rodrigues, A. | - |
dc.date.accessioned | 2012-05-18T11:26:07Z | - |
dc.date.available | 2012-05-18T11:26:07Z | - |
dc.date.issued | 2012-04 | - |
dc.identifier.issn | 0144-8617 | por |
dc.identifier.uri | https://hdl.handle.net/1822/19389 | - |
dc.description.abstract | about the influence of the extraction process on composition of the extracts and in ulvan itself. In this context, the two main objectives of the present work are (1) the establishment of an efficient extraction process for ulvan and (2) development of an accurate characterization methodology to evaluate the extract composition and ulvan content. Three ulvan-rich extracts obtained by different schemes of extraction were studied. The methodology for the analysis was improved and a detailed analysis of extracted ulvan was provided. The polysaccharide is rich in ulvanobiuronic acid 3-sulfate type A [→4)- -d-GlcAp-(1 → 4)- -l-Rhap 3S-(1→], with minor amounts of ulvanobiuronic acid 3-sulfate type B [→4)- -l-IdoAp-(1 → 4)- -l-Rhap 3S-(1→]. The extract with the higher degree of purification is a high molecular weight polysaccharide (790 kDa) composed of rhamnose (22.4%), glucuronic acid (22.5%), xylose (3.7%), iduronic acid (3.1%) and glucose (1.0%). It is highly sulfated (32.2%) and contains 1.3% of proteins and 10.3% of inorganic material. Applying simple extraction scheme it was possible to obtain an extract from green algae with high content of ulvan without affecting the overall chemical structure of the polysaccharide. | por |
dc.description.sponsorship | This work was supported by the project IBEROMARE ('Centro Multipolar de Valorizacao de Recursos e ResiduosMarinhos'), approved by Operational Programme for Cross-border Cooperation: Spain - Portugal, 2007-2013 (POCTEP), with funding contribution through the European Regional Development Fund (ERDF co-funding) and POCTEP.Anabela Alves is grateful for financial support from Fundacaopara a Ciencia e Tecnologia (FCT) through the SFRH/BD/39359/2007 grant.The authors thank Novozymes for kindly providing the enzymatic mixture. Authors are also grateful to Dra. Mariana Andrade and Materials Center of the University of Porto (CEMUP) for the acquisition of <SUP>13</SUP>C NMR spectra (NMR spectrometer is part of the National NMR Network and was purchased in the framework of the National Program for Scientific Re-equipment, contract REDE/1517/RMN/2005, with funds from POCI 2010 (FEDER) and FCT). | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.rights | restrictedAccess | por |
dc.subject | Ulvan | por |
dc.subject | Green algae | por |
dc.subject | Ulva lactuca | por |
dc.subject | Extraction | por |
dc.subject | Purification | por |
dc.subject | Polysaccharide characterization | por |
dc.title | Characterization of ulvan extracts to assess the effect of different steps in the extraction procedure | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 537 | por |
oaire.citationEndPage | 546 | por |
oaire.citationIssue | 2 | por |
oaire.citationTitle | Carbohydrate Polymers | por |
oaire.citationVolume | 88 | por |
dc.identifier.doi | 10.1016/j.carbpol.2011.12.041 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Carbohydrate Polymers | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Alves2012_CP.pdf Acesso restrito! | 575,74 kB | Adobe PDF | Ver/Abrir |