Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/19172
Título: | Development and characterization of a novel hybrid tissue engineering–based scaffold for spinal cord injury repair |
Autor(es): | Silva, Nuno Salgado, A. J. Sousa, R. A. Oliveira, J. T. Adriano, Pedro Leite-Almeida, Hugo Cerqueira, R. Almeida, Armando Mastronardi, F. Mano, J. F. Neves, N. M. Sousa, Nuno Reis, R. L. |
Palavras-chave: | Spinal cord injury Tissue engineering Biomaterials Neural regeneration |
Data: | 8-Mai-2012 |
Editora: | Mary Ann Liebert |
Revista: | Tissue Engineering : Part A |
Resumo(s): | Spinal cord injury (SCI) represents a significant health and social problem, and therefore it is vital to develop novel strategies that can specifically target it. In this context, the objective of the present work was to develop a new range of three-dimensional (3D) tubular structures aimed at inducing the regeneration within SCI sites. Up to six different 3D tubular structures were initially developed by rapid prototyping: 3D bioplotting–based on a biodegradable blend of starch. These structures were then further complemented by injecting Gellan Gum, a polysaccharide-based hydrogel, in the central area of structures. The mechanical properties of these structures were assessed using dynamic mechanical analysis, under both dry and wet conditions, and their morphologies/porosities were analyzed using micro-computed tomography and scanning electron microscopy. Biological evaluation was carried out to determine their cytotoxicity, using both minimum essential medium (MEM) extraction and MTS tests, as well as by encapsulation of an oligodendrocyte-like cell (M03-13 cell line) within the hydrogel phase. The histomorphometric analysis showed a fully interconnected network of pores with porosity ranging from 70% to 85%. Scaffolds presented compressive modulus ranging from 17.4 to 62.0 MPa and 4.42 to 27.4 MPa under dry and wet conditions, respectively. Cytotoxicity assays revealed that the hybrid starch/poly-ɛ-caprolactone/Gellan Gum scaffolds were noncytotoxic, as they did not cause major alterations on cell morphology, proliferation, and metabolic viability. Moreover, preliminary cell encapsulation assays showed that the hybrid scaffolds could support the in vitro culture of oligodendrocyte-like cells. Finally, preliminary in vivo studies conducted in a hemisection rat SCI model revealed that the above-referred structures were well integrated within the injury and did not trigger chronic inflammatory processes. The results herein presented indicate that these 3D systems might be of use in future SCI regeneration approaches. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/19172 |
ISSN: | 2152-4955 |
Versão da editora: | http://online.liebertpub.com/doi/abs/10.1089/ten.tea.2008.0559 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Silva_et al_TE10(1).pdf | Documento principal | 481,29 kB | Adobe PDF | Ver/Abrir |