Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/16843

TitleEfficacy of a broad host range lytic bacteriophage against E. coli adhered to urothelium
Author(s)Sillankorva, Sanna
Oliveira, D.
Moura, A.
Henriques, Mariana
Faustino, A.
Nicolau, Ana
Azeredo, Joana
Issue dateJul-2011
PublisherSpringer
JournalCurrent Microbiology
Abstract(s)Persistent urinary tract infections (UTI) are often caused by E. coli adhered to urothelium. This type of cells is generally recognized as very tolerant to antibiotics which renders difficult the treatment of chronic UTI. This work investigates the use of lytic bacteriophages as alternative antimicrobial agents, particularly the interaction of phages with E. coli adhered to urothelium and specifically determines their efficiency against this type of cells. The bacterial adhesion to urothelium was performed varying the bacterial cell concentrations and the period and conditions (static, shaken) of adhesion. Three collection bacteriophages (T1, T4 and phiX174 like phages) were tested against clinical E. coli isolates and only one was selected for further infection experiments. Based on the lytic spectrum against clinical isolates and its ability to infect the highest number of antibiotic resistant strains, the T1-like bacteriophage was selected. This bacteriophage caused nearly a 45 % reduction of the bacterial population after 2 h of treatment. This study provides evidence that bacteriophages are effective in controlling suspended and adhered cells and therefore can be a viable alternative to antibiotics to control urothelium adhered bacteria.
TypeArticle
URIhttp://hdl.handle.net/1822/16843
DOI10.1007/s00284-010-9834-8
ISSN0343-8651
Other identifiers0343-8651
1432-0991
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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