Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/16777

TitleApplication of flow cytometry for the identification of Staphylococcus epidermidis by peptide nucleic acid fluorescence in situ hybridization (PNA FISH) in blood samples
Author(s)Azevedo, N. F.
Jardim, T.
Almeida, Carina
Cerqueira, L.
Almeida, A. J.
Rodrigues, Fernando José dos Santos
Keevil, C. W.
Vieira, M. J.
KeywordsStaphylococcus epidermidis
Bloodstream infection
Flow cytometry
FISH
Nanodiagnostics
Permeabilization
Issue date2011
PublisherSpringer
JournalAntonie van Leeuwenhoek
Abstract(s)Staphylococcus epidermidis is considered to be one of the most common causes of nosocomial bloodstream infections, particularly in immune-compromised individuals. Here, we report the development and application of a novel peptide nucleic acid probe for the specific detection of S. epidermidis by fluorescence in situ hybridization. The theoretical estimates of probe matching specificity and sensitivity were 89 and 87%, respectively. More importantly, the probe was shown not to hybridize with closely related species such as Staphylococcus aureus. The method was subsequently successfully adapted for the detection of S. epidermidis in mixed-species blood cultures both by microscopy and flow cytometry.
Typearticle
URIhttp://hdl.handle.net/1822/16777
DOI10.1007/s10482-011-9595-9
ISSN0003-6072
1572-9699
Publisher versionhttp://www.springerlink.com/
Peer-Reviewedyes
AccessopenAccess
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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