Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/14041

TitleCombination of enzymes and flow perfusion conditions improves osteogenic differentiation of bone marrow stromal cells cultured upon starch/poly(å-caprolactone) fiber meshes
Author(s)Martins, Ana M.
Saraf, A.
Sousa, R. A.
Alves, C. M.
Mikos, Antonios G.
Kasper, F. Kurtis
Reis, R. L.
KeywordsStarch/poly(e-polycaprolactone) fiber meshes
a-amylase
Lipase
Flow perfusion bioreactor
Osteogenic
starch/poly(epsilon-polycaprolactone) fiber meshes
osteogenic differentiation
Starch/poly(ε-polycaprolactone) fiber meshes
Issue dateSep-2010
PublisherWiley
JournalJournal of Biomedical Materials Research Part A
Abstract(s)Previous studies have shown that a-amylase and lipase are capable of enhancing the degradation of fiber meshes blends of starch and poly(e-caprolactone) (SPCL) under dynamic conditions, and consequently to promote the proliferation and osteogenic differentiation of bone marrow stromal cells (MSCs). This study investigated the effect of flow perfusion bioreactor culture in combination with enzymes on the osteogenic differentiation of MSCs. SPCL fiber meshes were seeded with MSCs and cultured with osteogenic medium supplemented with a-amylase, lipase, or a combination of the two for 8 or 16 days using static or flow conditions. Lipase and its combination with a-amylase enhanced cell proliferation after 16 days. In addition, the flow perfusion culture enhanced the infiltration of cells and facilitated greater distribution of extracellular matrix (ECM) throughout the scaffolds in the presence/absence of enzymes. A significant amount of calcium was detected after 16 days in all groups cultured in flow conditions compared with static cultures. Nevertheless, when a-amylase and lipase were included in the flow perfusion cultures, the calcium content was 379 6 30 lg/scaffold after as few as 8 days. The highest calcium content (1271 6 32 lg/scaffold) was obtained for SPCL/cell constructs cultured for 16 days in the presence of lipase and flow. Furthermore, von Kossa staining and tetracycline fluorescence of histological sections demonstrated mineral deposition within the scaffolds for all groups cultured for 16 days under flow. However, all the data corroborate that lipase coupled with flow perfusion conditions improve the osteogenic differentiation of MSCs and enhance ECM mineralization.
TypeArticle
URIhttp://hdl.handle.net/1822/14041
DOI10.1002/jbm.a.32785
ISSN1552-4965
Peer-Reviewedyes
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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