Please use this identifier to cite or link to this item:

TitlePurification of polysaccharides from a biofilm matrix by selective precipitation of proteins
Author(s)Oliveira, Rosário
Marques, F.
Azeredo, Joana
KeywordsDifferential precipitation
Issue date1999
JournalBiotechnology Techniques
Citation"Biotechnology techniques" ISSN 0951-208X. 13:6 (1999) 391-393.
Series/Report no.1999;18
Abstract(s)The objective was to obtain the polysaccharide fraction presented in the extracted polymeric matrix of a biofilm by differential precipitation of the protein content. This was tried by pH adjustment and by addition of trichloroacetic acid (TCA). The best yield of protein precipitation was with 20% (w/v) TCA, with more than 97% of the protein removed and without affecting the amount of polysaccharides in solution.
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

Files in This Item:
File Description SizeFormat 
1999-18[1].pdf525,55 kBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID