Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/1383

TitlePurification of polysaccharides from a biofilm matrix by selective precipitation of proteins
Author(s)Oliveira, Rosário
Marques, F.
Azeredo, Joana
KeywordsDifferential precipitation
Polysaccharides
Proteins
Issue date1999
PublisherKluwer
JournalBiotechnology Techniques
Citation"Biotechnology techniques" ISSN 0951-208X. 13:6 (1999) 391-393.
Series/Report no.1999;18
Abstract(s)The objective was to obtain the polysaccharide fraction presented in the extracted polymeric matrix of a biofilm by differential precipitation of the protein content. This was tried by pH adjustment and by addition of trichloroacetic acid (TCA). The best yield of protein precipitation was with 20% (w/v) TCA, with more than 97% of the protein removed and without affecting the amount of polysaccharides in solution.
TypeArticle
URIhttp://hdl.handle.net/1822/1383
DOI10.1023/A:1008954301470
ISSN0951-208X
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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