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TitleEncapsulation of human articular chondrocytes into 3D hydrogel : phenotype and genotype characterization
Author(s)Pereira, Rui C.
Gentili, Chiara
Cancedda, Ranieri
Azevedo, Helena S.
Reis, R. L.
KeywordsNatural polymers
Chondrocyte encapsulation
Cell culture and survival within 3D matrix
Cell-based technology
Cartilage regeneration
Issue date2011
PublisherSpringer Science+Business Media
JournalMethods in Molecular Biology
Abstract(s)This chapter is intended to provide a summary of the current materials used in cell encapsulation technology as well as methods for evaluating the performance of cells encapsulated in a polymeric matrix. In particular, it describes the experimental procedure to prepare a hydrogel matrix based on natural polymers for encapsulating and culturing human articular chondrocytes with the interest in cartilage regeneration. Protocols to evaluate the viability, proliferation, differentiation, and matrix production of embedded cells are also described and include standard protocols such as the MTT and [3H] Thymidine assays, reverse transcription polymerase chain reaction (RT-PCR) technique, histology, and immunohistochemistry analysis. The assessment of cell distribution within the 3D hydrogel construct is also described using APoTome analysis.
TypeBook part
Publisher version
AccessOpen access
Appears in Collections:3B’s - Capítulos de Livros/Book Chapters

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