Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/12794

TitleGellan gum-based hydrogels for intervertebral disc tissue engineering applications
Author(s)Silva-Correia, Joana
Oliveira, Joaquim M.
Caridade, S. G.
Oliveira, João T.
Sousa, R. A.
Mano, J. F.
Reis, R. L.
KeywordsGellan gum
Hydrogels
Intervertebral disc
Methacrylation
Nucleus pulposus
Photo-crosslinking
Tissue engineering
Issue dateJun-2011
PublisherWiley-Blackwell
JournalJournal of Tissue Engineering and Regenerative Medicine
Abstract(s)Intervertebral disc (IVD) degeneration is a challenging clinical problem that urgently demands viable nucleus pulposus (NP) implant materials. The best suited biomaterial for NP regeneration has yet to be identified, but it is believed that biodegradable hydrogel-based materials are promising candidates. In this work, we have developed ionic- and photo-crosslinked methacrylated gellan gum (GG–MA) hydrogels to be used in acellular and cellular tissue-engineering strategies for the regeneration of IVDs. The physicochemical properties of the developed hydrogels were investigated by Fourier-transform infrared spectroscopy, 1H nuclear magnetic resonance and differential scanning calorimetry. The swelling ability and degradation rate of hydrogels were also analysed in phosphate-buffered saline solution at physiological pH for a period of 30 days. Additionally, the morphology and mechanical properties of the hydrogels were assessed under a scanning electron microscope and dynamic compression, respectively. An in vitro study was carried out to screen possible cytotoxicity of the gellan gum-based hydrogels by culturing rat lung fibroblasts (L929 cells) with hydrogel leachables up to 7 days. The results demonstrated that gellan gum was successfully methacrylated. We observed that the produced GG–MA hydrogels possess improved mechanical properties and lower water uptake ability and degradation rate as compared to gellan gum. This work also revealed that GG–MA hydrogels are non-cytotoxic in vitro, thus being promising biomaterials to be used in IVD tissue-engineering strategies.
TypeArticle
URIhttp://hdl.handle.net/1822/12794
DOI10.1002/term.363
ISSN1932-7005
Peer-Reviewedyes
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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