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https://hdl.handle.net/1822/11927
Título: | Evaluation antimicrobial and antiadhesive properties of the biosurfactant lunasan produced by candida sphaerica UCP 0995 |
Autor(es): | Luna, J. M. Rufino, R. D. Sarrubbo, L. A. Rodrigues, L. R. Teixeira, J. A. Takaki, G. M. Campos |
Palavras-chave: | bBosurfactant Antimicrobial activity Anti-adhesive activity |
Data: | Fev-2011 |
Editora: | Springer |
Revista: | Current Microbiology |
Citação: | "Current Microbiology". ISSN 0343-8651. 1:8 (Febr. 2011). |
Resumo(s): | Different groups of biosurfactants exhibit diverse properties and display a variety of physiological functions in producer microorganisms; these include enhancing the solubility of hydrophobic/water-insoluble compound, heave metal binding, bacterial pathogenesis, cell adhesion and aggregation, quorum sensing and biofilm formation. Candida sphaerica was grown in a low cost medium, consisting of distilled water supplemented with 9% refinery residue of soybean oil and 9% corn steep liquor, for 144 h at 28 C and 150 rpm. The cell-free supernatant obtained at the end of the experiments was submitted to extraction, and afterward the biosurfactant was isolated using methanol with a yield of 9 g l-1. The critical micelle concentration of the biosurfactant was found to be 0.25 mg ml-1 with a surface tension of 25 mN m-1. Several concentrations of the biosurfactant (0.625–10 mg ml-1) were used to evaluate its antimicrobial and antiadhesive activities against a variety of microorganisms. The biosurfactant showed antimicrobial activity against Streptococcus oralis (68%), Candida albicans (57%), and Staphylococcus epidermidis(57.6%) for the highest concentration tested. Furthermore, the biosurfactant at a concentration of 10 mg ml-1 inhibited the adhesion between 80 and 92% of Pseudomonas aeruginosa, Streptococcus agalactiae, Streptococcus sanguis12. Inhibition of adhesion with percentages near 100% occurred for the higher concentrations of biosurfactant used. Results gathered in this study point to a potential use of the biosurfactant in biomedical applications |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/11927 |
DOI: | 10.1007/s00284-011-9889-1 |
ISSN: | 0343-8651 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Luna_2011.pdf | Luna_2011 | 267,39 kB | Adobe PDF | Ver/Abrir |