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https://hdl.handle.net/1822/10586
Título: | Xanthone biosynthesis in Hypericum perforatum cells provides antioxidant and antimicrobial protection upon biotic stress |
Autor(es): | Franklin, Gregory Conceição, Luís F. R. Kombrink, E. Dias, Alberto Carlos Pires |
Palavras-chave: | Agrobacterium Hypericum perforatum Biotic stress Xanthones Antioxidant defense response Elicitation Phytoalexins |
Data: | 2009 |
Editora: | Elsevier 1 |
Revista: | Phytochemistry |
Citação: | “Phytochemistry”. ISSN 0031-9422. 70:1 (2009) 60-68. |
Resumo(s): | Xanthone production in Hypericum perforatum (HP) suspension cultures in response to elicitation by Agrobacterium tumefaciens co-cultivation has been studied. RNA blot analyses of HP cells co-cultivated with A. tumefaciens have shown a rapid up-regulation of genes encoding important enzymes of the general phenylpropanoid pathway (PAL, phenylalanine ammonia lyase and 4CL, 4-coumarate:CoA ligase) and xanthone biosynthesis (BPS, benzophenone synthase). Analyses of HPLC chromatograms of methanolic extracts of control and elicited cells (HP cells that were co-cultivated for 24 h with A. tumefaciens) have revealed a 12-fold increase in total xanthone concentration and also the emergence of many xanthones after elicitation. Methanolic extract of elicited cells exhibited significantly higher antioxidant and antimicrobial competence than the equivalent extract of control HP cells indicating that these properties have been significantly increased in HP cells after elicitation. Four major de novo synthesized xanthones have been identified as 1,3,6,7-tetrahydroxy-8-prenyl xanthone, 1,3,6,7-tetrahydroxy-2-prenyl xanthone, 1,3,7-trihydroxy-6-methoxy-8-prenyl xanthone and paxanthone. Antioxidant and antimicrobial characterization of these de novo xanthones have revealed that xanthones play dual function in plant cells during biotic stress: (1) as antioxidants to protect the cells from oxidative damage and (2) as phytoalexins to impair the pathogen growth. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/10586 |
DOI: | 10.1016/j.phytochem.2008.10.016 |
ISSN: | 0031-9422 |
Versão da editora: | www.elsevier.com/locate/phytochem |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Franklin et al 2009 Phytochem.pdf | Xanthone biosynthesis in Hypericum perforatum cells provides antioxidant and antimicrobial protection upon biotic stress | 482,36 kB | Adobe PDF | Ver/Abrir |