Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/10424
Título: | Pulsed electric field reduces the permeability of potato cell wall |
Autor(es): | Gómez Galindo, Federico Vernier, P. Thomas Dejmek, Petr Vicente, A. A. Gundersen, Martin A. |
Palavras-chave: | Cell wall FM1-43 eEectric fields Sress Oxidative cross-linking electric fields stress |
Data: | Mai-2008 |
Editora: | John Wiley and Sons |
Revista: | Bioelectromagnetics |
Citação: | "Bioelectromagnetic". ISSN 0197-8462. 29:4 (May 2008) 296-301. |
Resumo(s): | The effect of the application of pulsed electric fields to potato tissue on the diffusion of the fluorescent dye FM1-43 through the cell wall was studied. Potato tissue was subjected to field strengths ranging from 30 to 500 V/cm, with one 1 ms rectangular pulse, before application of FM1-43 and microscopic examination. Our results show a slower diffusion of FM1-43 in the electropulsed tissue when compared with that in the non-pulsed tissue, suggesting that the electric field decreased the cell wall permeability. This is a fast response that is already detected within 30 s after the delivery of the electric field. This response was mimicked by exogenous H2O2 and blocked by sodium azide, an inhibitor of the production of H2O2 by peroxidases. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/10424 |
DOI: | 10.1002/bem.20394 |
ISSN: | 0197-8462 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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2008-024.pdf | 152,75 kB | Adobe PDF | Ver/Abrir |