Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/10369
Registo completo
Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Madeira, Pedro | - |
dc.contributor.author | Teixeira, J. A. | - |
dc.contributor.author | Macedo, Eugénia | - |
dc.contributor.author | Mikheeva, Larissa M. | - |
dc.contributor.author | Zaslavsky, Boris Y. | - |
dc.date.accessioned | 2010-02-11T14:36:39Z | - |
dc.date.available | 2010-02-11T14:36:39Z | - |
dc.date.issued | 2008-05 | - |
dc.identifier.citation | "Journal of Chromatography A". ISSN 0021-9673. 1190:1-2 (May 2008) 39-43. | por |
dc.identifier.issn | 0021-9673 | por |
dc.identifier.uri | https://hdl.handle.net/1822/10369 | - |
dc.description.abstract | Distribution coefficients of randomly selected proteins were measured in aqueous two-phase systems (ATPSs) formed by different combinations of Dextran-75 (Dex), Ficoll-70, polyethylene glycol-8000 (PEG), hydroxypropyl starch-100 (PES), and Ucon50HB5100 (Ucon, a random copolymer of ethylene glycol and propylene glycol) at particular polymer concentrations, all containing 0.15 M NaCl in 0.01 M phosphate buffer, pH 7.4. Most of the proteins in the PEG-Ucon system precipitated at the interface. In the other ATPSs, namely, PES-PEG, PES-Ucon, Ficoll-PEG, Ficoll-Ucon, and in Dex-PEG and Dex-Ucon described earlier the distribution coefficients for the proteins were correlated according to the solvent regression equation: ln Ki = aio ln Ko + bio, where Ki and Ko are the distribution coefficients for any protein in the ith and oth two-phase systems. Coefficients aio and bio are constants, the values of which depend upon the particular compositions of the two-phase systems under comparison. | por |
dc.description.sponsorship | Fundação para a Ciência e a Tecnologia (FCT) | por |
dc.description.sponsorship | Fundo Europeu de Desenvolvimento Regional (FEDER) | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.relation | info:eu-repo/grantAgreement/FCT/POCI/60720/PT | - |
dc.rights | openAccess | por |
dc.subject | Aqueous two-phase systems | por |
dc.subject | Protein partitioning | por |
dc.subject | Collander equation | por |
dc.title | “On the collander equation”: protein partitioning in polymer/polymer aqueous two-phase systems | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
sdum.number | 1-2 | por |
sdum.pagination | 39-43 | por |
sdum.publicationstatus | published | por |
sdum.volume | 1190 | por |
oaire.citationStartPage | 39 | por |
oaire.citationEndPage | 43 | por |
oaire.citationIssue | 1-2 | por |
oaire.citationVolume | 1190 | por |
dc.identifier.doi | 10.1016/j.chroma.2008.03.003 | por |
dc.identifier.pmid | 18378246 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Journal of Chromatography A | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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2008-046.pdf | 375,2 kB | Adobe PDF | Ver/Abrir |