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https://hdl.handle.net/1822/92075
Título: | MiDAS 5: Global diversity of bacteria and archaea in anaerobic digesters |
Autor(es): | Dueholm, Morten Kam Dahl Andersen, Kasper Skytte Korntved, Anne-Kirstine C. Rudkjøbing, Vibeke Alves, M. M. Bajón-Fernández, Yadira Batstone, Damien Butler, Caitlyn Cruz, Mercedes Cecilia Davidsson, Åsa Erijman, Leonardo Holliger, Christof Koch, Konrad Kreuzinger, Norbert Lee, Changsoo Lyberatos, Gerasimos Mutnuri, Srikanth O'Flaherty, Vincent Oleskowicz-Popiel, Piotr Pokorna, Dana Rajal, Veronica Recktenwald, Michael Rodríguez, Jorge Saikaly, Pascal E. Tooker, Nick Vierheilig, Julia De Vrieze, Jo Wurzbacher, Christian Nielsen, Per Halkjær |
Palavras-chave: | Environmental biotechnology Environmental microbiology Metagenomics Microbial ecology |
Data: | 25-Jun-2024 |
Editora: | Springer Nature |
Revista: | Nature Communications |
Citação: | Dueholm, Morten Kam Dahl; Andersen, Kasper Skytte; Korntved, Anne-Kirstine C.; Rudkjøbing, Vibeke; Alves, M. Madalena; Bajón-Fernández, Yadira; Batstone, Damien; Butler, Caitlyn; Cruz, Mercedes Cecilia; Davidsson, Åsa; Erijman, Leonardo; Holliger, Christof; Koch, Konrad; Kreuzinger, Norbert; Lee, Changsoo; Lyberatos, Gerasimos; Mutnuri, Srikanth; O'Flaherty, Vincent; Oleskowicz-Popiel, Piotr; Pokorna, Dana; Rajal, Veronica; Recktenwald, Michael; Rodríguez, Jorge; Saikaly, Pascal E.; Tooker, Nick; Vierheilig, Julia; De Vrieze, Jo; Wurzbacher, Christian; Nielsen, Per Halkjær, MiDAS 5: Global diversity of bacteria and archaea in anaerobic digesters. Nature Communications, 15(5361), 2024 |
Resumo(s): | Anaerobic digestion of organic waste into methane and carbon dioxide (biogas) is carried out by complex microbial communities. Here, we use full-length 16S rRNA gene sequencing of 285 full-scale anaerobic digesters (ADs) to expand our knowledge about diversity and function of the bacteria and archaea in ADs worldwide. The sequences are processed into full-length 16S rRNA amplicon sequence variants (FL-ASVs) and are used to expand the MiDAS 4 database for bacteria and archaea in wastewater treatment systems, creating MiDAS 5. The expansion of the MiDAS database increases the coverage for bacteria and archaea in ADs worldwide, leading to improved genus- and species-level classification. Using MiDAS 5, we carry out an amplicon-based, global-scale microbial community profiling of the sampled ADs using three common sets of primers targeting different regions of the 16S rRNA gene in bacteria and/or archaea. We reveal how environmental conditions and biogeography shape the AD microbiota. We also identify core and conditionally rare or abundant taxa, encompassing 692 genera and 1013 species. These represent 8499% and 1861% of the accumulated read abundance, respectively, across samples depending on the amplicon primers used. Finally, we examine the global diversity of functional groups with known importance for the anaerobic digestion process. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/92075 |
DOI: | 10.1038/s41467-024-49641-y |
ISSN: | 2041-1723 |
Versão da editora: | https://www.nature.com/ncomms/ |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_57902_1.pdf | 2,03 MB | Adobe PDF | Ver/Abrir |