Utilize este identificador para referenciar este registo: https://hdl.handle.net/1822/91441

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dc.contributor.authorLima, Ângela Martinspor
dc.contributor.authorMuzny, Christina A.por
dc.contributor.authorCerca, Nunopor
dc.date.accessioned2024-05-21T16:05:47Z-
dc.date.available2024-05-21T16:05:47Z-
dc.date.issued2024-01-05-
dc.identifier.citationLima, Â.; Muzny, Christina A.; Cerca, Nuno, An indirect fluorescence microscopy method to assess vaginal Lactobacillus concentrations. Microorganisms, 12(1), 114, 2024por
dc.identifier.issn2076-2607por
dc.identifier.urihttps://hdl.handle.net/1822/91441-
dc.description.abstractLactobacillus species are the main colonizers of the vaginal microbiota in healthy women. Their absolute quantification by culture-based methods is limited due to their fastidious growth. Flow cytometry can quantify the bacterial concentration of these bacteria but requires the acquisition of expensive equipment. More affordable non-culturable methods, such as fluorescence microscopy, are hampered by the small size of the bacteria. Herein, we developed an indirect fluorescence microscopy method to determine vaginal lactobacilli concentration by determining the correlation between surface area bacterial measurement and initial concentration of an easily cultivable bacterium (Escherichia coli) and applying it to lactobacilli fluorescence microscopy counts. In addition, vaginal lactobacilli were quantified by colony-forming units and flow cytometry in order to compare these results with the indirect method results. The colony-forming-unit values were lower than the results obtained from the other two techniques, while flow cytometry and fluorescence microscopy results agreed. Thus, our developed method was able to accurately quantify vaginal lactobacilli.por
dc.description.sponsorshipFCT -Fundação para a Ciência e a Tecnologia(UIDB/04469/2020)por
dc.language.isoengpor
dc.publisherMDPI Publishingpor
dc.relationinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04469%2F2020/PTpor
dc.relation2022.13112.BDpor
dc.rightsopenAccesspor
dc.subjectBacterial quantificationpor
dc.subjectFlow cytometrypor
dc.subjectFluorescence microscopypor
dc.subjectColony forming unitspor
dc.subjectVaginal lactobacillipor
dc.titleAn indirect fluorescence microscopy method to assess vaginal Lactobacillus concentrationspor
dc.typearticle-
dc.peerreviewedyespor
dc.relation.publisherversionhttp://www.mdpi.com/journal/microorganismspor
dc.commentsCEB57739por
oaire.citationStartPage114por
oaire.citationIssue1por
oaire.citationConferencePlaceSwitzerland-
oaire.citationVolume12por
dc.date.updated2024-05-09T09:53:30Z-
dc.identifier.doi10.3390/microorganisms12010114por
dc.description.publicationversioninfo:eu-repo/semantics/publishedVersion-
sdum.journalMicroorganismspor
Aparece nas coleções:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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