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https://hdl.handle.net/1822/87272
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Campo DC | Valor | Idioma |
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dc.contributor.author | Carneiro, Mariana | por |
dc.contributor.author | Rodrigues, L. R. | por |
dc.contributor.author | Moreira, Felismina | por |
dc.contributor.author | Sales, M. G. F. | por |
dc.date.accessioned | 2023-11-20T11:10:38Z | - |
dc.date.issued | 2024 | - |
dc.identifier.citation | Carneiro, Mariana; Rodrigues, Lígia R.; Moreira, Felismina; Sales, M. G. F., Paper-based biomimetic test-strip for CA15-3 with coloured readout. Microchemical Journal, 196(109640), 2024 | por |
dc.identifier.issn | 0026-265X | por |
dc.identifier.uri | https://hdl.handle.net/1822/87272 | - |
dc.description.abstract | The combination of molecularly-imprinted polymers (MIPs) on paper substrates for capturing a cancer antigen 153 (CA15-3) with the traditional coloured transduction scheme of enzyme-linked immunosorbent assay (ELISA) assays using 3,3,5,5-tetramethylbenzidine (TMB), horseradish peroxidase (HRP) and H2O2 is presented here for the first time. Here, the paper surface was modified with a silane derivative containing an amine function that allows subsequent binding of 3-aminophenylboronic acid (3-APBA). The target protein, CA15-3, was then bound via the boronic groups of APBA. The empty space around CA15-3 was filled by polymerization of dopamine. Finally, the CA15-3 template was removed by breaking the imine function to create vacant sites for which CA15-3 has a high affinity. Binding of CA15-3 was detected by oxidation of TMB substrate by HRP in the presence of H2O2. The results showed that the MIP was able to selectively recognize CA15-3 within 3500 U mL-1, even in complex samples. Quantitative data were extracted by analysing the colour coordinates of images captured with a smartphone using ImageJ. This selective behaviour was confirmed by comparison with a control material (non polymer, NIP). Overall, the described approach has advantages over conventional ELISA, namely low cost and high stability, which is due to the use of MIP as a trapping element acting as a selective pre-concentration point of CA15-3. To our knowledge, this is the first biomimetic ELISA (B-ELISA) on paper substrate used for macromolecules such as proteins. We believe that this approach has the potential to be applied to other protein disease biomarkers, making it a suitable tool for screening glycoproteins at the point-of-care. | por |
dc.description.sponsorship | This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit. Authors also acknowledge FCT for funding the PhD grant reference SFRH/BD/131959/2017. | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04469%2F2020/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F131959%2F2017/PT | por |
dc.rights | restrictedAccess | por |
dc.subject | Molecularly imprinted polymer | por |
dc.subject | Biomimetic enzyme-linked immunosorbent assay | por |
dc.subject | Point-of-care | por |
dc.subject | Cellulose paper | por |
dc.subject | Cancer antigen 153 | por |
dc.subject | Protein biomarker | por |
dc.title | Paper-based biomimetic test-strip for CA15-3 with coloured readout | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S0026265X23012596?via%3Dihub | por |
dc.comments | CEB56505 | por |
oaire.citationIssue | 109640 | por |
oaire.citationConferencePlace | Netherlands | - |
oaire.citationVolume | 196 | por |
dc.date.updated | 2023-11-20T10:27:35Z | - |
dc.identifier.doi | 10.1016/j.microc.2023.109640 | por |
dc.date.embargo | 10000-01-01 | - |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
sdum.journal | Microchemical Journal | por |
oaire.version | P | - |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
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document_56505_1.pdf Acesso restrito! | 2,76 MB | Adobe PDF | Ver/Abrir |