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https://hdl.handle.net/1822/86887
Título: | Mitochondrial DNA D-loop amplification and sequencing for species differentiation in milk |
Autor(es): | Baptista, Marlene Domingues, Lucília |
Palavras-chave: | Species differentiation Milk adulteration Dairy authentication DNA extraction and purification Food quality Mitochondrial D-loop DNA |
Data: | 23-Ago-2023 |
Editora: | Springer |
Revista: | Methods in Molecular Biology |
Citação: | Baptista, M., Domingues, L. (2023). Mitochondrial DNA D-Loop Amplification and Sequencing for Species Differentiation in Milk. In: Domingues, L. (eds) PCR. Methods in Molecular Biology, vol 2967. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3358-8_14 |
Resumo(s): | Adulteration of dairy products, mainly through the substitution of high-quality milk for lower-quality milk, results in the production of low-value products, raising health, social, and economic concerns. As such, the development of methods to ensure dairy products' safety and quality is of great concern for governments and consumers. Although several methods have been developed for species differentiation in dairy products, their application and the establishment of reliable molecular markers for authentication purposes still need to be improved. In this chapter, we describe a low-cost, sensitive, fast, and reliable PCR-based method for mitochondrial D-loop DNA amplification for efficient detection of cattle milk in binary mixtures with sheep milk, thereby allowing the authentication of processed dairy products. |
Tipo: | Capítulo de livro |
URI: | https://hdl.handle.net/1822/86887 |
ISBN: | 978-1-0716-3357-1 |
e-ISBN: | 978-1-0716-3358-8 |
DOI: | 10.1007/978-1-0716-3358-8_14 |
ISSN: | 1064-3745 |
Versão da editora: | https://link.springer.com/protocol/10.1007/978-1-0716-3358-8_14 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
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document_56461_1.pdf Acesso restrito! | 193,93 kB | Adobe PDF | Ver/Abrir |