Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/86885| Título: | Gene expression quantification from pathogenic bacterial biofilms by quantitative PCR |
| Autor(es): | França, Ângela Maria Oliveira Sousa Cerca, Nuno |
| Palavras-chave: | Bacteria Biofilms Sample collection RNA isolation DNase treatment RNA quality Complementary DNA synthesis qPCR |
| Data: | 23-Ago-2023 |
| Editora: | Springer |
| Revista: | Methods in Molecular Biology |
| Citação: | França, A., Cerca, N. (2023). Gene Expression Quantification from Pathogenic Bacterial Biofilms by Quantitative PCR. In: Domingues, L. (eds) PCR. Methods in Molecular Biology, vol 2967. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3358-8_11 |
| Resumo(s): | Quantitative PCR (qPCR) is one of the most used techniques to quantify gene expression in bacterial biofilms due to its easiness, sensitivity, and robustness. However, several practical aspects need to be considered to obtain accurate and reliable results. Here, we describe a detailed and optimized protocol to quantify mRNA transcripts from bacterial biofilms using qPCR, including pieces of advice to improve RNA quality, which ultimately increases the accuracy, consistency, and relevance of gene expression data. |
| Tipo: | Capítulo de livro |
| URI: | https://hdl.handle.net/1822/86885 |
| ISBN: | 978-1-0716-3357-1 |
| e-ISBN: | 978-1-0716-3358-8 |
| DOI: | 10.1007/978-1-0716-3358-8_11 |
| ISSN: | 1064-3745 |
| Versão da editora: | https://link.springer.com/protocol/10.1007/978-1-0716-3358-8_11 |
| Arbitragem científica: | yes |
| Acesso: | Acesso restrito UMinho |
| Aparece nas coleções: |
Ficheiros deste registo:
| Ficheiro | Descrição | Tamanho | Formato | |
|---|---|---|---|---|
| document_56463_1.pdf Acesso restrito! | 287,26 kB | Adobe PDF | Ver/Abrir |
