Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/85295
Título: | Novel benzo[α]phenoxazinium chlorides functionalized with sulfonamide groups as NIR fluorescent probes for vacuole, endoplasmic reticulum, and plasma membrane staining |
Autor(es): | Ferreira, J. C. C. Sousa, Rui Pedro Carvalho Lima Preto, Ana Sousa, Maria João Gonçalves, M. Sameiro T. |
Palavras-chave: | Nile Blue Benzo[α]phenoxazines Sulfonamide groups NIR probes Fluorescent probes |
Data: | 3-Fev-2023 |
Editora: | Multidisciplinary Digital Publishing Institute (MDPI) |
Revista: | International Journal of Molecular Sciences |
Citação: | Ferreira, J.C.C.; Sousa, R.P.C.L.; Preto, A.; Sousa, M.J.; Gonçalves, M.S.T. Novel Benzo[α]phenoxazinium Chlorides Functionalized with Sulfonamide Groups as NIR Fluorescent Probes for Vacuole, Endoplasmic Reticulum, and Plasma Membrane Staining. Int. J. Mol. Sci. 2023, 24, 3006. https://doi.org/10.3390/ijms24033006 |
Resumo(s): | The demand for new fluorophores for different biological target imaging is increasing. Benzo[<i>a</i>]phenoxazine derivatives are fluorochromophores that show promising optical properties for bioimaging, namely fluorescent emission at the NIR of the visible region, where biological samples have minimal fluorescence emission. In this study, six new benzo[<i>a</i>]phenoxazinium chlorides possessing sulfonamide groups at 5-amino-positions were synthesized and their optical and biological properties were tested. Compared with previous probes evaluated using fluorescence microscopy, using different <i>S. cerevisiae</i> strains, these probes, with sulfonamide groups, stained the vacuole membrane and/or the perinuclear membrane of the endoplasmic reticulum with great specificity, with some fluorochromophores capable of even staining the plasma membrane. Thus, the addition of a sulfonamide group to the benzo[<i>a</i>]phenoxazinium core increases their specificity and attributes for the fluorescent labeling of cell applications and fractions, highlighting them as quite valid alternatives to commercially available dyes. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/85295 |
DOI: | 10.3390/ijms24033006 |
ISSN: | 1661-6596 |
e-ISSN: | 1422-0067 |
Versão da editora: | https://www.mdpi.com/1422-0067/24/3/3006 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CBMA - Artigos/Papers CDQuim - Artigos (Papers) |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
ijms-24-03006.pdf | 85,09 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons