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https://hdl.handle.net/1822/82948
Título: | Sfl1 is required for Candida albicans biofilm formation under acidic conditions |
Autor(es): | Gonçalves, Bruna Fernandes Barbosa, Ana Teresa Moutinho Soares, Ana Raquel Henriques, Mariana Silva, Sónia Carina |
Palavras-chave: | Candida biofilm Filamentation Acidic environment Sfl1 targets Regulation network Sfi1 targets |
Data: | Jun-2023 |
Editora: | Elsevier 1 |
Revista: | Biochimie |
Citação: | Gonçalves, Bruna; Barbosa, Ana; Soares, Ana Raquel; Henriques, Mariana; Silva, Sónia Carina, Sfl1 is required for Candida albicans biofilm formation under acidic conditions. Biochimie, 209, 37-43, 2023 |
Resumo(s): | Candida albicans is a common Candida species, responsible for infections in various anatomical sites under different environmental conditions, aggravated in the presence of its biofilms. As such, this study aimed to reveal the regulation of C. albicans biofilms under acidic conditions by the transcription factor Sfl1, whose role on biofilm formation is unclear. For that, microbiologic and transcriptomic analyses were performed with the knock-out mutant C. albicans sfl1/sfl1 and its parental strain SN76, grown in planktonic and biofilm lifestyles at pH 4 (vaginal pH). The results revealed that despite being a filamentation repressor Sf1 is required for maximal biofilm formation under acidic conditions. Additionally, Sfl1 was found to induce 275 and 126 genes in biofilm and planktonic cells, respectively, with an overlap of 19 genes. The functional distribution of Sfl1 targets was similar in planktonic and biofilm modes but an enrichment of carbohydrate metabolism function was found in biofilm cells, including some genes encoding proteins involved in the biofilm matrix production. Furthermore, this study shows that the regulatory network of Sfl1 in acidic biofilms is complex and include the positive and negative regulation of transcription factors involved in adhesion and biofilm formation, such as AHR1, BRG1, TYE7, TEC1, WOR1, and various of their targets. Overall, this study shows that Sfl1 is a relevant regulator of C. albicans biofilm formation in acidic environments and contributes to a better understanding of C. albicans virulence under acidic conditions. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/82948 |
DOI: | 10.1016/j.biochi.2023.01.011 |
ISSN: | 0300-9084 |
Versão da editora: | https://www.sciencedirect.com/journal/biochimie |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_56039_1.pdf Acesso restrito! | 1,6 MB | Adobe PDF | Ver/Abrir |