Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/79865
Título: | Squaraine dyes as serum albumins probes: synthesis, photophysical experiments and molecular docking studies |
Autor(es): | Gomes, Vanessa Sofia Dias Boto, Renato E. F. Almeida, Paulo Coutinho, Paulo J. G. Pereira, Mário R. Gonçalves, M. Sameiro T. Reis, Lucinda V. |
Palavras-chave: | Barbituric acid derivatives Barbiturate squaraine dyes Fluorescence probes NIR fluorescence dyes Serum albumins |
Data: | 28-Jul-2021 |
Editora: | Elsevier 1 |
Revista: | Bioorganic Chemistry |
Citação: | Vanessa S.D. Gomes, Renato E.F. Boto, Paulo Almeida, Paulo J.G. Coutinho, Mário Rui Pereira, M. Sameiro T. Gonçalves, Lucinda V. Reis, Squaraine dyes as serum albumins probes: Synthesis, photophysical experiments and molecular docking studies, Bioorganic Chemistry, Volume 115, 2021, 105221, ISSN 0045-2068, https://doi.org/10.1016/j.bioorg.2021.105221. |
Resumo(s): | Three barbiturate squaraine dyes derived from indolenine or benzothiazole, with different barbituric acid derivatives were prepared, characterized and photophysically evaluated by standard spectroscopic methods. As expectable for squaraines, these dyes showed narrow and intense absorption and emission bands in the Vis/NIR region. The interaction of synthesized dyes with bovine and human serum albumins (BSA and HSA) was also evaluated in phosphate buffer (PB). The results revealed that upon the addition of BSA or HSA the complex dye-protein emit more fluorescence, and the emission intensity is directly proportional to the concentration of protein used (0–3.5 µM). The titration tests allowed to calculate the binding constants, in an order of magnitude of 104-106 M, as well as the limits of detection and quantification in the nanomolar tens range. All dyes showed a good response to the interaction with both proteins, but the most pronounced envisioning their use as protein labeling was observed for the squaraine dye derived from the indolenine with a 1,3-dimethylbarbituric acid moiety. The molecular docking studies revealed the existence of a binding between the compounds and four sites on the HSA molecule, where one of these four locations is a new binding site with which this series of dye interacts. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/79865 |
DOI: | 10.1016/j.bioorg.2021.105221 |
ISSN: | 0045-2068 |
Versão da editora: | https://www.sciencedirect.com/science/article/pii/S0045206821005988 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | PHYSICS OF QUANTUM MATERIALS AND BIONANOSTRUCTURES (2018 - ...) |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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1-s2.0-S0045206821005988-main.pdf Acesso restrito! | 6,12 MB | Adobe PDF | Ver/Abrir |