Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/78388
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Costa, Susana Patrícia Chaves | por |
dc.contributor.author | Cunha, Alexandra Maria Pinto | por |
dc.contributor.author | Freitas, Paulo P. | por |
dc.contributor.author | Carvalho, Carla Alexandra Oliveira Cunha Medeiros | por |
dc.date.accessioned | 2022-06-14T10:01:07Z | - |
dc.date.available | 2022-06-14T10:01:07Z | - |
dc.date.issued | 2022-06 | - |
dc.identifier.citation | Costa, Susana P.; Cunha, Alexandra; Freitas, Paulo P.; Carvalho, Carla M., A phage receptor-binding protein as a promising tool for the detection of Escherichia coli in human specimens. Frontiers in Microbiology, 13(871855), 2022 | por |
dc.identifier.uri | https://hdl.handle.net/1822/78388 | - |
dc.description.abstract | Escherichia coli is a problematic pathogen that causes life-threatening diseases, being a frequent causative agent of several nosocomial infections such as urinary tract and bloodstream infections. Proper and rapid bacterial identification is critical for allowing prompt and targeted antimicrobial therapy. (Bacterio)phage receptor-binding proteins (RBPs) display high specificity for bacterial surface epitopes and, therefore, are particularly attractive as biorecognition elements, potentially conferring high sensitivity and specificity in bacterial detection. In this study, we elucidated, for the first time, the potential of a recombinant RBP (Gp17) to recognize E. coli at different viability states, such as viable but not culturable cells, which are not detected by conventional techniques. Moreover, by using a diagnostic method in which we combined magnetic and spectrofluorimetric approaches, we demonstrated the ability of Gp17 to specifically detect E. coli in various human specimens (e.g., whole blood, feces, urine, and saliva) in about 1.5 h, without requiring complex sample processing. | por |
dc.description.sponsorship | This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the project Phages-on-chip PTDC/BTM-SAL/32442/2017 (POCI-01-0145- FEDER-032442) and the strategic funding of the research units CEB (UIDB/04469/2020) and INESC MN (UID/05367/2020) through the pluriannual BASE and PROGRAMATICO financing and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020, Programa Operacional Regional do Norte. SC was supported by the FCT grant SFRH/BD/130098/2017. | por |
dc.language.iso | eng | por |
dc.publisher | Frontiers Media | por |
dc.relation | info:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FBTM-SAL%2F32442%2F2017/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04469%2F2020/PT | por |
dc.relation | UID/05367/2020 | por |
dc.relation | info:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F130098%2F2017/PT | por |
dc.rights | openAccess | por |
dc.subject | Healthcare-associated infections | por |
dc.subject | Cell viability states | por |
dc.subject | Diagnostic method | por |
dc.subject | Human biological samples | por |
dc.subject | Receptor-binding proteins | por |
dc.subject | Escherichia coli | por |
dc.title | A phage receptor-binding protein as a promising tool for the detection of Escherichia coli in human specimens | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://journal.frontiersin.org/journal/microbiology | por |
dc.comments | CEB55591 | por |
oaire.citationStartPage | 1 | por |
oaire.citationEndPage | 14 | por |
oaire.citationVolume | 13 | por |
dc.date.updated | 2022-06-13T16:39:37Z | - |
dc.identifier.eissn | 1664-302X | por |
dc.identifier.doi | 10.3389/fmicb.2022.871855 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
dc.subject.wos | Science & Technology | por |
sdum.journal | Frontiers in Microbiology | por |
dc.identifier.articlenumber | 871855 | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_55591_1.pdf | 2,51 MB | Adobe PDF | Ver/Abrir |