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https://hdl.handle.net/1822/77044
Título: | Thymbra capitata essential oil has a significant antimicrobial activity against methicillin-resistant Staphylococcus aureus pre-formed biofilms |
Autor(es): | Almeida, Luciana Lopes, Nathalie Gaio, Vânia Silva Cavaleiro, Carlos Salgueiro, Lígia Silva, Vanessa Poeta, Patrícia Cerca, Nuno |
Palavras-chave: | MRSA EO minimum inhibitory concentration (MIC) minimum lethal concentration (MLC) biofilm biomass cell culturability cellular activity XTT minimum inhibitory concentration minimum lethal concentration |
Data: | 6-Fev-2022 |
Editora: | Wiley-Blackwell |
Revista: | Letters in Applied Microbiology |
Citação: | Almeida, Luciana; Lopes, Nathalie; Gaio, Vania; Cavaleiro, Carlos; Salgueiro, Lígia; Silva, Vanessa; Poeta, Patrícia; Cerca, Nuno, Thymbra capitata essential oil has a significant antimicrobial activity against methicillin-resistant Staphylococcus aureus pre-formed biofilms. Letters in Applied Microbiology, 2022 |
Resumo(s): | Abstract Methicillin-resistant Staphylococcus aureus (MRSA) is a multidrug-resistant opportunistic pathogen with a great ability to form biofilms. Herein, the antimicrobial potential of Thymbra capitata essential oil (EO) against MRSA biofilms was investigated. The determination of the minimum inhibitory concentration (MIC) and the minimum lethal concentration (MLC) of the T. capitata EO was first investigated on a group of clinical isolates from septicemias, diabetic foot ulcers and osteomyelitis. Biofilms were incubated with the EO at the MLC and its anti-biofilm potential was investigated. A strong antimicrobial activity was observed, with MIC and MLC values between 0.32 and 0.64 mg L-1. However, the concentration of EO necessary for the eradication of planktonic cells was insufficient to significantly reduce the biofilm biomass of some isolates. Nevertheless, cell culturability and overall cellular metabolism was strongly reduced in all biofilms tested, only when the EO was tested. Contrary to the tested antibiotics, T. capitata EO showed a significant antimicrobial activity against MRSA biofilms, by reducing cellular metabolism and cellular culturability. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/77044 |
DOI: | 10.1111/lam.13665 |
ISSN: | 0266-8254 |
Versão da editora: | http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1472-765X |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_55269_1.pdf Acesso restrito! | 8,14 MB | Adobe PDF | Ver/Abrir |