Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/74496
Título: | Flow-FISH using nucleic acid mimic probes for the detection of bacteria |
Autor(es): | Azevedo, Andreia S. Rocha, Rui Dias, Nicolina Marques |
Palavras-chave: | Peptide nucleic acid Locked nucleic acid 2'O-methyl RNA Nucleic acid mimics Hybridization Fixation Permeabilization Flow cytometry |
Data: | 13-Fev-2021 |
Editora: | Springer |
Revista: | Methods in Molecular Biology |
Citação: | Azevedo, Andreia S.; Rocha, Rui; Dias, Nicolina M., Flow-FISH using nucleic acid mimic probes for the detection of bacteria. In Nuno F. Azevedo, Carina Almeida, Fluorescence In-Situ Hybridization (FISH) for Microbial Cells: Methods and Concepts, Vol. Methods in Molecular Biology 2246, Springer US, 2021. ISBN: 978-1-0716-1115-9, 263-277 |
Resumo(s): | Flow-Fluorescence in situ hybridization (Flow-FISH) enables multiparametric high-throughput detection of target nucleic acid sequences at the single cell-level, allowing an accurate quantification of different cell populations by using a combination of flow cytometry and fluorescent in situ hybridization (FISH). In this chapter, a flow-FISH protocol is described with labeled nucleic acid mimics (NAMs) (e.g. LNA/2OMe and PNA) acting as the reporter molecules. This protocol allows for the specific detection of bacterial cells. Hence, this protocol can be carried out with minor adjustments, in order to simultaneously detect different species of bacteria in different types of clinical, food, or environmental samples. |
Tipo: | Capítulo de livro |
URI: | https://hdl.handle.net/1822/74496 |
ISBN: | 978-1-0716-1115-9 |
DOI: | 10.1007/978-1-0716-1115-9_17 |
ISSN: | 1064-3745 |
Versão da editora: | https://link.springer.com/book/10.1007/978-1-0716-1115-9 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
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document_54200_1.pdf Acesso restrito! | 317,5 kB | Adobe PDF | Ver/Abrir |