Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/73639
Título: | Partial coated stem cells with bioinspired silica as new generation of cellular hybrid materials |
Autor(es): | Maciel, Marta M. Correia, Tiago R. Gaspar, Vítor M. Rodrigues, João M. M. Choi, Insung S. Mano, J. F. |
Palavras-chave: | Advanced cell-material interactions Cell partial coatings Cell-based materials Organicinorganic hybrid composites Solgel processes advanced cell‐ material interactions cell‐ based materials organic– inorganic hybrid composites sol– gel processes |
Data: | 16-Jul-2021 |
Editora: | Wiley-Blackwell |
Revista: | Advanced Functional Materials |
Citação: | Maciel, Marta; Correia, Tiago R.; Gaspar, Vítor M.; Rodrigues, João M. M.; Choi, Insung S.; Mano, João F., Partial coated stem cells with bioinspired silica as new generation of cellular hybrid materials. Advanced Functional Materials, 31(29), 2009619, 2021 |
Resumo(s): | The manipulation of cell surfaces in anchorage-dependent cells can help overcome difficulties presented during cell handling and application. Silica-based protective mechanisms existing in free-floating microorganisms may be used as inspiration for sustaining human cell survival in suspension. Gathering on this, herein human adipose-derived mesenchymal stem cells (hASCs) are partially coated with a hard silica layer that operates as a supporting platform for individual cells in suspension. Inspired by the organic templates involved in biosilicification, a novel chitosan (CHT) derivative displaying fully natural quaternary amine moieties is synthetized via a rapid, one-pot strategy. Silicification is promoted on individual hASCs surfaces via a two-step process: a priming step onto previously adhered cell with this CHT derivative, followed by a biocompatible solgel process. hASCs holding a silica backpack exhibit enhanced cell survival in suspension conditions and can spread and acquire a more adherent phenotype. This new protocol for cell-surface modification also provides a new generation of hybrid materials with functionalization of the silica backpack, which can be applied to different areas such as tissue engineering, biosensing, drug delivery, and targeted cell-based therapies. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/73639 |
DOI: | 10.1002/adfm.202009619 |
ISSN: | 1616-301X |
e-ISSN: | 1616-3028 |
Versão da editora: | https://onlinelibrary.wiley.com/doi/full/10.1002/adfm.202009619 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_54279_1.pdf Acesso restrito! | 3,14 MB | Adobe PDF | Ver/Abrir |