Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/73430
Título: | Electrochemistry-assisted surface plasmon resonance biosensor for detection of CA 153 |
Autor(es): | Ribeiro, José A. Sales, Maria Goreti F. Pereira, Carlos M. |
Palavras-chave: | Immunology Sensors Peptides and proteins Surface plasmon resonance Biopolymers |
Data: | Mai-2021 |
Editora: | American Chemical Society |
Revista: | Analytical Chemistry |
Citação: | Ribeiro, José; Sales, M. G. F.; Pereira, Carlos M., Electrochemistry-assisted surface plasmon resonance biosensor for detection of CA 153. Analytical Chemistry, 93(22), 7815-7824, 2021 |
Resumo(s): | In this work, we describe an innovative methodology based on combined surface plasmon resonance (SPR) and electrochemical responses (eSPR) in the same immunoassay for screening CA 153 cancer biomarker with high sensitivity (and selectivity), in a very simple, label-free, accurate, and fully automated manner. Detection was achieved by performing two simple steps. In the first step, direct SPR was used to monitor CA 153 interaction with surface immobilized antibody. Two linear response ranges were obtained and the detection limit achieved is poor (LOD of 21 U mL1). However, in the second detection step, electrochemical measurements at the SPR gold surface were performed to measure the decrease of redox probe peak current upon antigenantibody interaction, providing a suitable amplification strategy to lower detection levels of CA 153 (LOD of 0.0998 U mL1), without the need of additional complex and/or expensive amplification steps to enhance the sensitivity. Moreover, selectivity studies were performed against other common cancer biomarkers and the results showed that the eSPR immunosensor is selective for the CA 153 protein. Finally, the clinical applicability of the developed eSPR biosensing methodology was successfully applied to detect CA 153 in human serum samples at clinically relevant levels due to the high sensitivity of electrochemical readout. The same concept may be further extended to other proteins of interest. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/73430 |
DOI: | 10.1021/acs.analchem.0c05367 |
ISSN: | 1520-6882 |
e-ISSN: | 0003-2700 |
Versão da editora: | http://pubs.acs.org/journal/ancham |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_54465_1.pdf Acesso restrito! | 1,13 MB | Adobe PDF | Ver/Abrir |