Utilize este identificador para referenciar este registo: https://hdl.handle.net/1822/73054

Registo completo
Campo DCValorIdioma
dc.contributor.authorSousa-Silva, M.por
dc.contributor.authorRibas, David Manuel Nogueirapor
dc.contributor.authorCasal, Margaridapor
dc.contributor.authorSoares-Silva, Isabel Joãopor
dc.date.accessioned2021-05-28T15:53:49Z-
dc.date.available2021-05-28T15:53:49Z-
dc.date.issued2018-06-
dc.identifier.urihttps://hdl.handle.net/1822/73054-
dc.description.abstractThe wide applicability of organic acids for direct use as commodity chemicals and as polymer building blocks has evidenced their importance into diverse types of industries. As organic acids, carboxylic acids (CAs) are underlined as top value products by the US Department of Energy (DOE), which has included nine organic acids among the future top 12 value-added platform chemicals. In the yeast Saccharomyces cerevisiae, two permeases are responsible for the uptake of carboxylates at the plasma membrane. With affinity for lactate, pyruvate acetate and propionate, the JEN1 gene encodes a monocarboxylate proton symporter that belongs to the Major Facilitator Superfamily (TC 2.A.1.12.2). The ADY2 gene encodes an acetate permease, a member of the Acetate Uptake Transporter (AceTr) Family (TC 2.A.96.1.4). In the yeast Cyberlindnera jadinii, a close relative of Candida utilis, different uptake systems for CAs were functionally characterized however until now the genes encoding these transporters remain unidentified. In this work, carboxylic acid transporter homolog genes from C. jadinii were identified and expressed in Saccharomyces cerevisiae. The C. jadinii ScJEN1 and ScADY2 homologs were identified through sequence alignment, secondary structure prediction and phylogenetic analysis of putative transporters. In C. jadinii, 6 genes homolog to ScJEN1 (Cjj23088, Cjj21966, Cjj22358, Cjj21989, Cjj21602, Cjj25129) and 4 genes homolog to ScADY2 (Cja24587, Cja20823, Cja20690, Cja20822) were identified. These genes were expressed under the control of a GPD constitutive promoter, in a S. cerevisiae jen1Δady2Δ strain, that presents no activity for plasma membrane carboxylate permeases. GFP-fusions versions were performed revealing the localization of C. jadinii ScJEN1 and ScADY2 homolog transporters mainly at the plasma membrane. The complete characterization of these new plasma membrane transporters in C. jadinii is ongoing.por
dc.description.sponsorshipPTDC/BIAMIC/5184/2014. NORTE-01-0145-FEDER-000009. NORTE-08-5369-FSE-000060por
dc.language.isoengpor
dc.relationinfo:eu-repo/grantAgreement/FCT/5876/147364/PTpor
dc.rightsopenAccesspor
dc.titleFunctional characterization of Cyberlindnera jadinii carboxylate transporters in Saccharomyces cerevisiaepor
dc.typepanelPresentationpor
dc.peerreviewedyespor
oaire.citationStartPage77por
oaire.citationConferencePlaceBraga, Portugalpor
dc.subject.fosCiências Naturais::Ciências Biológicaspor
sdum.conferencePublicationXXI Jornadas de Biologia de Leveduras "Professor Nicolau van Uden"por
oaire.versionAMpor
Aparece nas coleções:CBMA - Resumos em livros de atas/Abstracts in proceedings

Ficheiros deste registo:
Ficheiro Descrição TamanhoFormato 
Abstracts jornadas 2018 7_6_2018 pdf.pdf921,26 kBAdobe PDFVer/Abrir

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID